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Series GSE7005 Query DataSets for GSE7005
Status Public on Feb 14, 2007
Title Transcriptional Profile of Lactobacillus helveticus grown in milk vs. MRS medium
Platform organism Lactobacillus helveticus CNRZ32
Sample organism Lactobacillus helveticus
Experiment type Expression profiling by array
Summary L. helveticus is used to modulate cheese flavor and as a starter organism in certain cheese varieties. Our group has compiled a draft (4x) sequence for the 2.4 Mb genome of an industrial strain L. helveticus CNRZ32. The primary aim was to investigate expression of 168 completely sequenced genes during growth in milk and MRS medium using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays where the non-interrupted sequence contigs were covered by consecutive 24-mer probes.
Keywords: growth conditions response
Overall design Milk and MRS cultures were incubated at 42oC until cells reached mid-log phase. Three independent growth experiments in milk and MRS were done; two identical samples were taken from each culture and used for the RNA isolation. A total of six RNA samples for each growth condition were used to independently produce labeled cDNA for microarray experiments.
Contributor(s) Smeianov VV, Wechter P, Broadbent JR, Hughes JE, Rodríguez BT, Christensen TK, Ardö Y, Steele JL
Citation(s) 17322329
Submission date Feb 10, 2007
Last update date Mar 16, 2012
Contact name James Steele
Organization name UW-Madison
Department Food Science
Street address 1605 Linden Dr.
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL4817 NimbleGen Lactobacillus helveticus CNRZ32 169/tiling
Samples (12)
GSM161537 L. helveticus CNRZ32 milk1.1
GSM161572 L. helveticus CNRZ32 milk1.2
GSM161573 L. helveticus CNRZ32 milk2.1
BioProject PRJNA99253

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7005_RAW.tar 80.1 Mb (http)(custom) TAR (of DATA, PAIR)

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