|
| Status |
Public on Jun 27, 2015 |
| Title |
Protein kinase C is essential for viability of the rice blast fungus Magnaporthe oryzae |
| Organism |
Pyricularia oryzae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A strain of M. oryzae was constructed in which the native PKC1 gene was replaced with an analogue-sensitive PKC1 allele (pkc1AS). This allele could be inhbited by the analogue 1NA-PP1. Global transcriptional profiling of mycelium following selective PKC inhibition identified significant changes in gene expression associated with cell wall re-modelling, autophagy, signal transduction and secondary metabolism.
|
| |
|
| Overall design |
Mycelium was grown in the presence and absence (control) of the inhibitor 1NA-PP1 for a number of different times up to 24 hours.
|
| |
|
| Contributor(s) |
Penn TJ, Soanes DM, Talbot NJ |
| Citation(s) |
26192090 |
| |
| Submission date |
Jun 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Darren Mark Soanes |
| E-mail(s) |
d.m.soanes@exeter.ac.uk
|
| Organization name |
University of Exeter
|
| Department |
College of Medicine and Health
|
| Street address |
RILD Building, RD&E Hospital Wonford, Barrack Road
|
| City |
Exeter |
| ZIP/Postal code |
EX2 5DW |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL16530 |
Illumina HiSeq 2000 (Magnaporthe oryzae) |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA288294 |
| SRA |
SRP059911 |
Less...
More...