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Series GSE70308 Query DataSets for GSE70308
Status Public on Jun 27, 2015
Title Protein kinase C is essential for viability of the rice blast fungus Magnaporthe oryzae
Organism Pyricularia oryzae
Experiment type Expression profiling by high throughput sequencing
Summary A strain of M. oryzae was constructed in which the native PKC1 gene was replaced with an analogue-sensitive PKC1 allele (pkc1AS). This allele could be inhbited by the analogue 1NA-PP1. Global transcriptional profiling of mycelium following selective PKC inhibition identified significant changes in gene expression associated with cell wall re-modelling, autophagy, signal transduction and secondary metabolism.
 
Overall design Mycelium was grown in the presence and absence (control) of the inhibitor 1NA-PP1 for a number of different times up to 24 hours.
 
Contributor(s) Penn TJ, Soanes DM, Talbot NJ
Citation(s) 26192090
Submission date Jun 26, 2015
Last update date May 15, 2019
Contact name Darren Mark Soanes
E-mail(s) d.m.soanes@exeter.ac.uk
Organization name University of Exeter
Department College of Medicine and Health
Street address RILD Building, RD&E Hospital Wonford, Barrack Road
City Exeter
ZIP/Postal code EX2 5DW
Country United Kingdom
 
Platforms (1)
GPL16530 Illumina HiSeq 2000 (Magnaporthe oryzae)
Samples (22)
GSM1723518 T0_REP1
GSM1723519 T0_REP2
GSM1723520 T1_POS_REP1
Relations
BioProject PRJNA288294
SRA SRP059911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70308_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
GSE70308_pkc_expression_all.txt.gz 732.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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