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| Status |
Public on Jul 08, 2015 |
| Title |
Chromosomal origin utilisation in Haloferax meiditerranei wild type and mutant strains |
| Organism |
Haloferax mediterranei |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
We have comprehensively explored the origin utilisation in Haloferax mediterranei. Here we report three active chromosomal origins by genome-wide replication profiling, and demonstrate that when these three origins are deleted, a dormant origin becomes activated.
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| Overall design |
Genomic DNA was extracted from the H. mediterranei cultures at specific time points(12h,18h,24h,36h,42h and stationary phase 66h), or during the exponential phase (OD600≈0.5) and stationary phase (OD600≈4.0)for the origin deletion strains. After being lablled, Genomic DNA from exponential phase and stationary phase were hybridized to Haloferax mediterranei genome array genechips.
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| Contributor(s) |
Yang H, Wu Z, Liu J, Wang L, Liu X, Xiang H |
| Citation(s) |
26374389 |
| |
| Submission date |
Jul 07, 2015 |
| Last update date |
Oct 09, 2015 |
| Contact name |
Haibo Yang |
| Organization name |
Institute of Microbiology, Chinese Academy of Sciences
|
| Street address |
No.1 Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| GSM1811391 |
DF50 genomic DNA of 12h vs that of stationary phase |
| GSM1811392 |
DF50 genomic DNA of 18h vs that of stationary phase |
| GSM1811393 |
DF50 genomic DNA of 24h vs that of stationary phase |
| GSM1811394 |
DF50 genomic DNA of 36h vs that of stationary phase |
| GSM1811395 |
DF50 genomic DNA of 42h vs that of stationary phase |
| GSM1811396 |
DF50ΔoriC1 genomic DNA of exponential phase vs that of stationary phase |
| GSM1811397 |
DF50ΔoriC2 genomic DNA of exponential phase vs that of stationary phase |
| GSM1811398 |
DF50ΔoriC3 genomic DNA of exponential phase vs that of stationary phase |
| GSM1811399 |
DF50ΔoriC1ΔoriC3 genomic DNA of exponential phase vs that of stationary phase |
| GSM1811400 |
DF50ΔoriC1ΔoriC2ΔoriC3 genomic DNA of exponential phase vs that of stationary phase |
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| Relations |
| BioProject |
PRJNA289136 |
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