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Series GSE70931 Query DataSets for GSE70931
Status Public on Sep 13, 2015
Title Tc17 cells are a pro-inflammatory, plastic lineage of pathogenic CD8+ T-cells that induce GVHD without anti-leukemic effects
Organism Mus musculus
Experiment type Expression profiling by array
Summary IL-17-producing cells are important mediators of graft-versus-host disease (GVHD) after allogeneic stem cell transplantation (SCT). Here we demonstrate that a distinct CD8+ Tc17 population develops rapidly after SCT but fails to maintain lineage fidelity such that they are unrecognizable in the absence of a fate reporter. Tc17 differentiation is dependent on alloantigen presentation by host-DC together with IL-6. Tc17 cells express high levels of multiple prototypic lineage-defining transcription factors (e.g. RORgt, T-bet) and cytokines (e.g. IL-17A, IL-22, IFNg, GM-CSF, IL-13). Targeted depletion of Tc17 early after transplant protects from lethal acute GVHD, however Tc17 cells are non-cytolytic and fail to mediate graft–versus–leukemia (GVL) effects. Thus, the Tc17 differentiation program during GVHD culminates in a highly plastic, hyper-inflammatory, poorly-cytolytic effector population which we term inflammatory Tc17 (iTc17). Since iTc17 mediate GVHD without contributing to GVL, therapeutic inhibition of iTc17 development in a clinical setting represents an attractive approach for separating GVHD and GVL.
 
Overall design Single colour, Illumina MouseRef-8 v2.0 Beadarrays.
 
Contributor(s) Gartlan KH, Markey KA, Varelias A, Bunting MD, Koyama M, Kuns RD, Raffelt NC, Olver SD, Lineburg KE, Cheong M, Teal BE, Lor M, Comerford I, Teng MW, Smyth MJ, McCluskey J, Rossjohn J, Stockinger B, Boyle GM, Lane SW, Clouston AD, McColl SR, MacDonald KP, Hill GR
Citation(s) 26206951
Submission date Jul 15, 2015
Last update date Jun 14, 2018
Contact name Glen M Boyle
E-mail(s) Glen.Boyle@qimr.edu.au
Phone +61 7 3362 0319
Organization name QIMR Berghofer Medical Research Insitute
Department Department of Cell and Molecular Biology, Cancer Program
Lab Cancer Drug Mechanisms Group
Street address 300 Herston Road Herston
City Brisbane
State/province Queensland
ZIP/Postal code 4006
Country Australia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1822045 CD8+YFP+ Replicate 1
GSM1822046 CD8+YFP+ Replicate 2
GSM1822047 CD8+YFP+ Replicate 3
Relations
BioProject PRJNA289964

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70931_RAW.tar 3.1 Mb (http)(custom) TAR
GSE70931_non-normalized.txt.gz 878.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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