NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE71196 Query DataSets for GSE71196
Status Public on Dec 17, 2015
Title Identification and characterization of MCF01A circular RNAs [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Circular RNAs (circRNAs) are widespread circular forms of non-coding RNAs with largely unknown function. Because stimulation of mammary cells with the epidermal growth factor (EGF) leads to dynamic changes in the abundance of both coding and non-coding RNA molecules, and culminates in the acquisition of a robust migratory phenotype, this cellular model might disclose functions of circRNAs. Here we show that circRNAs of EGF-stimulated mammary cells are stably expressed, while mRNAs and micro-RNAs change within minutes. In general, the circRNAs we detected are relatively long-lived and weakly expressed. Interestingly, they are almost ubiquitously co-expressed with the corresponding linear transcripts, and the respective, shared promoter regions are more active compared to genes producing linear isoforms only. These findings imply that altered abundance of circRNAs, unlike changes in the levels of other RNAs, might not play critical roles in signaling cascades and downstream transcriptional networks that rapidly commit cells to specific outcomes.
 
Overall design Histone 3 Lysine 27 Acetylation – 2 replicates
 
Contributor(s) Enuka Y
Citation(s) 26657629
Submission date Jul 22, 2015
Last update date May 15, 2019
Contact name Yehoshua Enuka
E-mail(s) ys.enuka@gmail.com
Phone +972-54-2345623
Organization name Weizmann Institute of Science
Department Biological Regulation
Lab Yarden
Street address Herzl 234
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (1)
GSM1829629 H3K27Ac_ChIPSeq
This SubSeries is part of SuperSeries:
GSE71197 Identification and characterization of MCF01A circular RNAs
Relations
BioProject PRJNA290558
SRA SRP061406

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71196_RAW.tar 45.3 Mb (http)(custom) TAR (of TSV, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap