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Series GSE7160 Query DataSets for GSE7160
Status Public on Apr 23, 2007
Title Distinct roles of HDAC complexes in promoter silencing, antisense suppression and DNA damage protection
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by genome tiling array
Genome binding/occupancy profiling by genome tiling array
Summary Histone acetylation plays an important role in regulating DNA accessibility. Multifunctional Sin3 proteins bind histone deacetylases (HDACs) to assemble silencing complexes that selectively target chromatin. We show that, in fission yeast, an essential HDAC Clr6 exists in two distinct Sin3 core complexes. Complex I contains an essential Sin3 homologue Pst1 and other factors, and predominantly targets gene promoters. Complex II contains a non-essential Sin3, Pst2, and several conserved proteins. It preferentially targets transcribed chromosomal regions and centromere cores. Defects in complex II abrogate global protective functions of chromatin, causing increased accessibility of DNA to genotoxic agents and widespread antisense transcripts that are processed by the exosome. Interestingly, the two Clr6 complexes differentially repress forward and reverse centromeric repeat transcripts, suggesting that these complexes regulate transcription in heterochromatin and euchromatin in a similar manner, including suppression of spurious transcripts from cryptic start sites.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series
Web link
Contributor(s) Nicolas E, Yamada T, Cam HP, FitzGerald PC, Kobayashi R, Grewal SI
Citation(s) 17450151
Submission date Feb 28, 2007
Last update date Dec 28, 2012
Contact name Shiv Grewal
Phone 2407607553
Organization name NCI
Department LBMB
Lab Shiv Grewal
Street address NCI bldg 37 Rm 6068 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL4682 pombe 44K_v2
Samples (29)
GSM162913 K9ac_1_WT
GSM162926 K9ac_2_WT
GSM162927 K9ac_1_alp13D
This SuperSeries is composed of the following SubSeries:
GSE7042 ChIP-chip analyses of H3K9 acetylation in WT, alp13 deletion and pst1-1 mutant S.pombe cells.
GSE7113 ChIP-chip analyses of H3K9 methylation in alp13 deletion and clr6-1 mutant S.pombe cells.
GSE7136 ChIP-chip analyses of H3K9 methylation in rrp6 deletion and rrp6 alp13 double mutant S.pombe cells.
BioProject PRJNA98163

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7160_RAW.tar 283.7 Mb (http)(custom) TAR (of TXT)

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