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Series GSE71730 Query DataSets for GSE71730
Status Public on Oct 01, 2015
Title Plasma induced signatures reveal an extracellular milieu possessing an immunoregulatory bias in treatment naïve inflammatory bowel disease
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The complex milieu of inflammatory mediators associated with many diseases is often too dilute to directly measure in the periphery, necessitating development of more sensitive measurements suitable for mechanistic studies, earlier diagnosis, guiding selection of therapy, and monitoring interventions. Previously, we determined that plasma of recent-onset (RO) Type 1 diabetes (T1D) patients induce a proinflammatory transcriptional signature in fresh peripheral blood mononuclear cells (PBMC) relative to that of unrelated healthy controls (HC). Here, using an optimized cryopreserved PBMC-based protocol, we apply this approach to inflammatory bowel disease by examining groups of Crohn's disease (CD) and ulcerative colitus (UC) patients. The induced plasma induced signatures are compared to those of Type 1 diabetes patients (RO T1D) as well as unrelated healthy controls (uHC).
 
Overall design UPN727 cells were stimulated with CD plasma (n=22), UC plasma (n=15), Unrelated healthy control (n=10). Gene expression analysis was perfromed in order to evaluate the transcriptional signature associated with IBD.
 
Contributor(s) Gurram B, Salzman NH, Kaldunski ML, Jia S, Stephens M, Sood MR, Hessner MJ
Citation(s) 26660358
Submission date Aug 04, 2015
Last update date Mar 25, 2019
Contact name Martin Hessner
E-mail(s) mhessner@mcw.edu
Organization name Medical College of Wisconsin
Department Pediatrics
Lab Max McGee National Research Center for Juvenile Diabetes
Street address 8701 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (47)
GSM1844252 UPN727 cells, unrelated healthy control (uHC) plasma patients 01
GSM1844253 UPN727 cells, unrelated healthy control (uHC) plasma patients 02
GSM1844254 UPN727 cells, unrelated healthy control (uHC) plasma patients 03
Relations
BioProject PRJNA291926

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71730_RAW.tar 197.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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