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Series GSE71907 Query DataSets for GSE71907
Status Public on Aug 10, 2016
Title RNA-seq analysis determined the effect of pat or cobB mutation on transcriptional levels in Salmonella Typhimurium 14028s.
Organism Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028
Experiment type Expression profiling by high throughput sequencing
Summary Salmonella causes a range of diseases in different hosts, including enterocolitis and systemic infection. Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown.
Reversible acetylation in Salmonella Typhimurium depends on acetyltransferase Pat and nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase CobB.
Here, we used cell and animal models to evaluate the virulence of pat and cobB deletion mutants in S. Typhimurium, and found that pat is essential for bacterial intestinal colonization, systemic infection and host inflammation response.
Next, to understand the underlying mechanism, genome-wide transcriptome was analyzed. RNA-seq data showed the expression of Salmonella pathogenicity islands 1 (SPI-1) is partially dependent on pat. In addition, we found that HilD is a substrate of Pat, which is essential for maintaining HilD protein level.
Taken together, these results suggested that protein acetylation system regulates SPI-1 expression by controlling HilD in a post-translational manner to mediate S. Typhimurium virulence.
 
Overall design To use RNA-seq to analyze the transcriptome patterns of pat or cobB mutation in Salmonella Typhimurium 14028s.
 
Contributor(s) Sang Y, Ren J, Lu J, Yao Y
Citation(s) 26810370
Submission date Aug 10, 2015
Last update date May 15, 2019
Contact name Yu Sang
E-mail(s) sshaobo2008@126.com
Phone +86 021 63846590 776534
Organization name Shanghai Jiao Tong University School of Medicine
Department Microbiology and Immunology
Street address 280 S. Chongqing Rd
City Shanghai
ZIP/Postal code 200025
Country China
 
Platforms (1)
GPL20792 Illumina HiSeq 2000 (Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028)
Samples (3)
GSM1847296 1_wild type
GSM1847297 2_pat mutant
GSM1847298 3_cobB mutant
Relations
BioProject PRJNA292430
SRA SRP062232

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Supplementary file Size Download File type/resource
GSE71907_RAW.tar 693.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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