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Series GSE72224 Query DataSets for GSE72224
Status Public on Nov 21, 2019
Title Age-Related Changes in the Transcriptome of Antibody-Secreting Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We analyzed age-related defects in B cell populations from young and aged mice. Microarray analysis of bone marrow resident antibody secreting cells (ASCs) showed significant changes upon aging, affecting multiple genes, pathways and functions including those that play a role in immune regulation, humoral immune responses, chromatin structure and assembly, cell metabolism and the endoplasmic reticulum (ER) stress response. Aged ASCs expressed higher levels of immunoregulators including PD-1. Increased PD-1 expression was also seen on mature aged B cells indicating early defects in the lineage. High PD-1 expression on the aged ASCs correlated with high cellular reactive oxygen species levels. Mitochondrial defects in aged B cells were further confirmed by the absence of an increases in basal respiration observed upon in vitro stimulation of young B cells
Overall design B cells from young and old mice were isolated and assayed using Illumina Mouse 6v2 arrays to find transcriptional changes upone aging
Web link
Contributor(s) Hildegund E, Senthil K, Noor D
Citation(s) 26967249
Submission date Aug 20, 2015
Last update date Feb 20, 2020
Contact name Louise C Showe
Phone 215-898-3791
Organization name The Wistar Institute
Lab Dr. Louise Showe
Street address 3601 Spruce St
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (17)
GSM1858081 Young.1
GSM1858082 Young.2
GSM1858083 Young.3.1
BioProject PRJNA293415

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72224_RAW.tar 15.8 Mb (http)(custom) TAR
GSE72224_non-normalized.txt.gz 5.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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