|
| Status |
Public on Nov 21, 2019 |
| Title |
Age-Related Changes in the Transcriptome of Antibody-Secreting Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We analyzed age-related defects in B cell populations from young and aged mice. Microarray analysis of bone marrow resident antibody secreting cells (ASCs) showed significant changes upon aging, affecting multiple genes, pathways and functions including those that play a role in immune regulation, humoral immune responses, chromatin structure and assembly, cell metabolism and the endoplasmic reticulum (ER) stress response. Aged ASCs expressed higher levels of immunoregulators including PD-1. Increased PD-1 expression was also seen on mature aged B cells indicating early defects in the lineage. High PD-1 expression on the aged ASCs correlated with high cellular reactive oxygen species levels. Mitochondrial defects in aged B cells were further confirmed by the absence of an increases in basal respiration observed upon in vitro stimulation of young B cells
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| |
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| Overall design |
B cells from young and old mice were isolated and assayed using Illumina Mouse 6v2 arrays to find transcriptional changes upone aging
|
| Web link |
https://www.ncbi.nlm.nih.gov/pubmed/26967249
|
| |
|
| Contributor(s) |
Hildegund E, Senthil K, Noor D |
| Citation(s) |
26967249 |
| |
| Submission date |
Aug 20, 2015 |
| Last update date |
Feb 20, 2020 |
| Contact name |
Louise C Showe |
| E-mail(s) |
lshowe@wistar.org
|
| Phone |
215-898-3791
|
| Organization name |
The Wistar Institute
|
| Lab |
Dr. Louise Showe
|
| Street address |
3601 Spruce St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA293415 |
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