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Series GSE72520 Query DataSets for GSE72520
Status Public on Aug 05, 2017
Title Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism
Organism Mus musculus
Experiment type Expression profiling by array
Summary The neuroectoderm is patterned along a rostral-caudal axis in response to localized factors in the embryo, but exactly how these factors act as positional information for this patterning is not yet fully understood. Here, using the self-organizing properties of mouse embryonic stem cell (ESC), we report that ESC-derived neuroectoderm self-generates a Six3+ rostral and a Irx3+ caudal bipolarized patterning. In this instance, localized Fgf signaling performs dual roles, as it regulates Six3+ rostral polarization at an earlier stage and promotes Wnt signaling at a later stage. The Wnt signaling components are differentially expressed in the polarized tissues, leading to genome-wide Irx3+ caudal-polarization signals. Surprisingly, differentially expressed Wnt agonists and antagonists have essential roles in orchestrating the formation of a balanced rostral-caudal neuroectoderm pattern. Together, our findings provide key processes for dynamic self-patterning and evidence that a temporally and locally regulated interaction between Fgf and Wnt signaling controls self-patterning in ESC-derived neuroectoderm.
Overall design The duplicated samples for each of Rax::GFP+ and Rax::GFP- cells during ESC (embryonic stem cell)-derived neural tissue development were analyzed.
Contributor(s) Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y
Citation(s) 29109536
Submission date Aug 31, 2015
Last update date Feb 11, 2019
Contact name NOZOMU TAKATA
Organization name Northwestern University Feinberg School of Medicine
Department Center for Vascular and Developmental Biology
Street address 303 East Superior Street, 10-220
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM1863994 Rax_GFP+_rep1
GSM1863995 Rax_GFP+_rep2
GSM1863996 Rax_GFP-_rep1
BioProject PRJNA294258

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72520_RAW.tar 14.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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