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Series GSE73015 Query DataSets for GSE73015
Status Public on Sep 15, 2015
Title Stable inhibitory activity of regulatory T cells requires the transcription factor Helios
Organism Mus musculus
Experiment type Expression profiling by array
Summary The maintenance of immune homeostasis requires regulatory T cells (Tregs). Given their intrinsic self-reactivity, Tregs must stably maintain a suppressive phenotype to avoid autoimmunity. We report that impaired expression of the transcription factor (TF) Helios by FoxP3+ CD4 and Qa-1-restricted CD8 Tregs results in defective regulatory activity and autoimmunity in mice. Helios-deficient Treg develop an unstable phenotype during inflammatory responses characterized by reduced FoxP3 expression and increased effector cytokine expression secondary to diminished activation of the STAT5 pathway. CD8 Treg also require Helios-dependent STAT5 activation for survival and to prevent terminal T cell differentiation. Definition of Helios as a key transcription factor that stabilizes regulatory T-cells in the face of inflammatory responses provides a genetic explanation for a core property of regulatory T-cells.
Overall design We used microarrays to detail the global programs of gene expression by CD8 Treg (CD44+CD122+Ly49+) and conventional memory type of CD8 cells (CD44+CD122+Ly49-).
Contributor(s) Kim HJ, Kreslavsky T, Cantor H
Citation(s) 26472910
Submission date Sep 14, 2015
Last update date Feb 11, 2019
Contact name Hye-Jung Kim
Organization name Dana Farber Cancer Institute
Department Cancer Immunology and Virology
Lab Cantor
Street address 450 Brookline
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM1880770 Ly49+ CD8 cells, rep1
GSM1880771 Ly49- CD8 cells, rep1
GSM1880772 Ly49+ CD8 cells, rep2
BioProject PRJNA295971

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73015_RAW.tar 17.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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