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Series GSE73124 Query DataSets for GSE73124
Status Public on Apr 13, 2016
Title Genome wide profiling of the RNAPII, γH2AX, H3K4Me1, H3K4M2 and H3K4Me3 in MLL2 F/F and FC/FC MEF cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary We investigated the RNAPII and γH2AX occupancy genome wide by ChIP-Seq in MLL2 F/F and FC/FC80 MEF cells. We found that a week after MLL2 excision (FC/FC cells), a group of genes present higher levels of γH2AX and RNAPII near the TSS, as compared to the control (F/F cells). H3K4Me1, H3K4M2 and H3K4Me3 levels near the TSS were also studied.
 
Overall design There is a total of 52 samples. 3 independent replicates for each experiment were performed. H3, H2AX and IgG ChIPs were used for normalisation or as controls.The experiments were performed using immortalised mouse embryonic fibroblasts (MEF) in which both MLL2 alleles were targeted by the loxp system (F/F cells). Tamoxifen treatment of the F/F cells for 24 hours results in the excision of both MLL2 alleles (FC/FC cells).
 
Contributor(s) Kantidakis T, Svejstrup JQ
Citation(s) 26883360
Submission date Sep 17, 2015
Last update date May 15, 2019
Contact name Theo Kantidakis
E-mail(s) theo.kantidakis@crick.ac.uk
Phone 01707625838
Organization name Francis Crick Institute
Department Clare Hall Laboratories
Lab Svejstrup Lab
Street address Blanche Lane
City South Mimms
ZIP/Postal code EN6 3LD
Country United Kingdom
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (52)
GSM1887355 gH2AX.FC/FC_NGS-3394
GSM1887356 gH2AX.FC/FC_NGS-3406
GSM1887357 gH2AX.FC/FC_NGS-6273
This SubSeries is part of SuperSeries:
GSE73130 Mutation of cancer driver MLL2 results in transcription stress and genome instability
Relations
BioProject PRJNA296118
SRA SRP063844

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73124_RAW.tar 90.3 Mb (http)(custom) TAR (of BED, RPKM, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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