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Series GSE73326 Query DataSets for GSE73326
Status Public on Jan 01, 2017
Title DNA Methylation analysis of polybrominated diphenyl ethers (PBDEs) treated hepatocellular carcinoma
Organism Mus musculus
Experiment type Methylation profiling by genome tiling array
Summary DE-71, a commercial mixture of the polybrominated diphenyl ethers (PBDEs), is used as flame retardant and the potential exposure to PBDEs is an emerging public health concern. It has been clarified that long term exposure to PBDEs induces hepatocellular carcinoma in mouse. In this study, we analyzed how DE-71 impacts on DNA methylation in hepatocellular carcinoma.
 
Overall design DE-71 (technical pentabromodiphenyl oxide; CAS. No. 32534-81-9) was obtained from Great Lakes Chemical Corporation (West Lafayette, IN - Lot 2550OA30A). The chemical was administered to male B6C3F1/N mice (Taconic Farms, Inc. (Germantown, NY) by oral gavage in corn oil 5 days per week at doses of 100 mg/kg/day for up to 18 months. Frozen liver tissue/tumors were collected for DNA extraction at necropsy of control and high dose mice at 18 months. Methylated CpG Island Recovery Assay (MIRA) was used.
 
Contributor(s) Shimbo T, Wade P, Mav D, Shah R
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Submission date Sep 22, 2015
Last update date Jan 02, 2017
Contact name Deepak Mav
Organization name Sciome LLC
Street address 2 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL16158 NimbleGen Mus musculus 100718_MM8_Deluxe_Promoter_HX1 array
Samples (8)
GSM1890850 DE-71 treated tumor rep1
GSM1890851 DE-71 treated tumor rep2
GSM1890852 DE-71 treated non-tumor rep1
Relations
BioProject PRJNA296683

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73326_RAW.tar 812.8 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table

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