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Series GSE73838 Query DataSets for GSE73838
Status Public on Sep 30, 2016
Title The Protein Non-anchored cell wall protein NCW-1 promotes cellulase production through effects on cellobiose uptake in Neurospora crassa
Organism Neurospora crassa
Experiment type Expression profiling by high throughput sequencing
Summary The mechanism of cellulase induction in the filamentous fungi is still not completely understood, and the components of this pathway are not well characterized. In this study, the mechanism of how the non-anchored cell wall protein NCW-1 (NCU05137) affect the cellulases induction was investigated. Transcriptome analysis of this quadruple deletion strain △3βG△ncw-1 showed that the expression of major cellulase, cellodextrin transporters and cellulase regulators were significantly increased. Taken together, the cellulase expression machinery was dramatically stimulated in Δ3βGΔncw-1. These results make us understanding the ncw-1 function better and deepened our knowledge about the cellulase induction in N. crassa.
 
Overall design △3βG△ncw-1 and △3βG were precultured for 20 h in MM, and then transferred into Vogel’s salts containing 1% cellobiose for an additional 4 h. Total RNA from frozen samples was extracted and treated.
 
Contributor(s) Lin L, Tian C, Li J
Citation(s) 28039555
Submission date Oct 08, 2015
Last update date May 15, 2019
Contact name liangcai lin
E-mail(s) lin_lc@tib.cas.cn
Organization name Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
Street address 32 West 7th Avenue, Tianjin Airport Economic Area
City Tianjin
ZIP/Postal code 300308
Country China
 
Platforms (1)
GPL16164 Illumina HiSeq 2000 (Neurospora crassa)
Samples (2)
GSM1904310 △3βG Cellobiose
GSM1904311 △3βG△ncw-1 Cellobiose
Relations
BioProject PRJNA298147
SRA SRP064599

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Supplementary file Size Download File type/resource
GSE73838_BG123_vs_S22_RPKM.txt.gz 136.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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