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Series GSE73841 Query DataSets for GSE73841
Status Public on Mar 01, 2016
Title The purine metabolite allantoin can activate the jasmonate signaling pathway in a MYC2-regulated and ABA-dependent manner
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Allantoin is a metabolic intermediate of purine catabolism that often accumulates in stressed plants. Recently, using Arabidopsis knockout mutants (aln) of ALLANTOINASE, we showed that this purine metabolite activates ABA production, thereby stimulating stress-related gene expression and enhancing seedling tolerance to abiotic stress. A detailed re-examination of the microarray data of an aln mutant (aln-1) not only confirmed increased expression of ABA-inducible genes, but also revealed altered expression of genes involved in jasmonic acid (JA) responses, likely under the control of MYC2, a master switch in the JA signaling pathway. Consistent with the transcriptome profiles, the aln-1 mutant displayed increased JA levels and enhanced responses to mechanical wounding and exogenous JA. Moreover, aln mutants demonstrated modestly increased susceptibility to hemibiotrophic and necrotrophic pathogens, probably reflecting the antagonistic action of MYC2 on the defense against these bacteria. Exogenously administered allantoin elicited the expression of JA-responsive genes including MYC2 in wild-type plants, supporting that allantoin might be responsible for the observed JA-related aln phenotypes. However, the effect of exogenous allantoin was suppressed by mutations deficient in bioactive JA (jar1-1), insensitive to JA (myc2-3) and deficient in ABA (aba2-1 and bglu18). The suppressive effect of jar1-1 and bglu18 mutations was further confirmed in the aln-1 background (jar1-1/aln-1 and bglu18/aln-1). These results indicate that allantoin can activate the MYC2-regulated JA signaling pathway through ABA production. Overall, this study provides evidence for the possible connection of purine catabolism with stress hormone homeostasis and signaling, and highlights the importance of allantoin in these interactions.
Evidence has been presented only recently for the involvement of purine catabolism in stress protection of plants and the mechanism behind this remains obscure. Here we show that in Arabidopsis, the intermediary metabolite allantoin can activate the MYC2-regulated jasmonate signaling pathway via the mechanism involving ABA, providing the link between the metabolism and two interactive signaling pathways of stress hormones that play critical roles in plant adaptation to environmental adversity.
Overall design Two replicates of the mutant were compared with controls. This series is a re-analysis of GSE44922.
Contributor(s) Takagi H, Ishiga Y, Egusa M, Watanabe S, Konishi T, Akiyoshi N, Matsuura T, Mori IC, Hirayama T, Kaminaka H, Shimada H, Sakamoto A
Citation(s) 26931169
Submission date Oct 08, 2015
Last update date Jun 12, 2017
Contact name Tomokazu Konishi
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (4)
GSM1904333 aln-1 mutant, replicate 1
GSM1904334 aln-1 mutant, replicate 2
GSM1904335 control, replicate 1
Reanalysis of GSE44922
BioProject PRJNA298207

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73841_RAW.tar 8.0 Mb (http)(custom) TAR (of CEL)
GSE73841_normalized_data_with_pvalues.txt.gz 764.4 Kb (ftp)(http) TXT
Processed data included within Sample table

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