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Series GSE74165 Query DataSets for GSE74165
Status Public on Apr 24, 2016
Title Dnmt3a regulates myeloproliferation and liver-specific homing and expansion of hematopoietic stem and progenitor cells (Bisulfite-Seq)
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary DNMT3A mutations are observed in myeloid malignancies, including myeloproliferative neoplasms (MPN), myelodysplastic syndromes (MDS), and acute myeloid leukemia (AML). Here we investigated the impact of conditional hematopoietic Dnmt3a loss on disease phenotype in primary mice. Dnmt3a ablation led to a lethal, fully penetrant myeloproliferative neoplasm with myelodysplasia (MDS/MPN) characterized by marked, progressive hepatomegaly that was transplantable. We detected expanded stem/progenitor populations in the liver of Dnmt3a-ablated mice. Homing studies showed that Dnmt3a-deleted bone marrow cells preferentially migrated to the liver. Hence, in addition to the established role of Dnmt3a in regulating self-renewal, Dnmt3a regulates tissue tropism and limits myeloid progenitor expansion in vivo.
 
Overall design Dnmt3af/f mice (Nguyen et al) were crossed into hematopoietic-specific inducible Mx1-Cre deletor line; we examined transcriptomes from FACS-sorted LSK and GMP populations from Dnmt3af/f Mx1-Cre+ (KO) and Dnmt3af/f Mx1-Cre- (CTRL) animals at 12 months of age
 
Contributor(s) Guryanova OA, Spitzer B, Garrett-Bakelman F, Levine RL
Citation(s) 26710888
Submission date Oct 19, 2015
Last update date May 15, 2019
Contact name Barbara Spitzer
E-mail(s) spitzerb@mskcc.org
Organization name Memorial Sloan Kettering Cancer Center
Lab Levine Lab
Street address 411 E 67th St
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (11)
GSM1912525 cKO_Mx_pos_GMP_178 ERRBS
GSM1912526 cKO_Mx_pos_GMP_179 ERRBS
GSM1912527 cKO_Cre_neg_GMP_346 ERRBS
This SubSeries is part of SuperSeries:
GSE74166 Dnmt3a regulates myeloproliferation and liver-specific homing and expansion of hematopoietic stem and progenitor cells
Relations
BioProject PRJNA299197
SRA SRP064984

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74165_RAW.tar 170.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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