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| Status |
Public on Apr 11, 2019 |
| Title |
Human pluripotency is initiated and preserved by a unique subset of founder cells [microarray] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The assembly of organized colonies is the earliest manifestation in the derivation or induction of pluripotency in vitro. However, the necessity and origin of this assemblance is unknown. Here, we identify human pluripotent founder cells (hPFCs) that initiate as well as preserve and establish pluripotent stem cell (PSC) cultures. PFCs are marked by N-cadherin expression (NCAD+) and reside exclusively at the colony boundary of primate PSCs. As demonstrated by functional analysis, hPFCs harbor the clonogenic capacity of PSC cultures and emerge prior to commitment events or phenotypes associated with pluripotent reprogramming. Comparative single cell analysis with pre and post implantation primate embryos revealed hPFCs share hallmark properties with primitive endoderm (PrE) and can be regulated by non-canonical Wnt signaling. Uniquely informed by primate embryo organization in vivo, our study defines a subset of founder cells critical to the establishment pluripotent state.
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| Overall design |
Gene-expression profiles of FACS-isolated H9 hESC subsets (NCAD+, NCAD-:SSEA3+, and NCAD-:SSEA3-) by microarray (Affymetrix Human Gene 1.0 ST Array). The FACS-isolation and total RNA extraction were repeated three times independently.
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| Contributor(s) |
Nakanishi M, Bhatia M |
| Citation(s) |
30982595 |
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| Submission date |
Nov 13, 2015 |
| Last update date |
Feb 05, 2021 |
| Contact name |
Mickie Bhatia |
| E-mail(s) |
mbhatia@mcmaster.ca
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| Organization name |
McMaster University
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| Department |
Stem Cell and Cancer Research Institute (SCC-RI)
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| Street address |
1200 Main Street West
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| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8N 3Z5 |
| Country |
Canada |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM1940016 |
H9 embryonic stem cells, Ncad negative SSEA3 negative sorted population, biological rep1 |
| GSM1940017 |
H9 embryonic stem cells, Ncad negative SSEA3 negative sorted population, biological rep2 |
| GSM1940018 |
H9 embryonic stem cells, Ncad negative SSEA3 negative sorted population, biological rep3 |
| GSM1940019 |
H9 embryonic stem cells, Ncad negative SSEA3 positive sorted population, biological rep1 |
| GSM1940020 |
H9 embryonic stem cells, Ncad negative SSEA3 positive sorted population, biological rep2 |
| GSM1940021 |
H9 embryonic stem cells, Ncad negative SSEA3 positive sorted population, biological rep3 |
| GSM1940022 |
H9 embryonic stem cells, Ncad positive sorted population, biological rep1 |
| GSM1940023 |
H9 embryonic stem cells, Ncad positive sorted population, biological rep2 |
| GSM1940024 |
H9 embryonic stem cells, Ncad positive sorted population, biological rep3 |
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| Relations |
| BioProject |
PRJNA302155 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74990_RAW.tar |
39.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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