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Series GSE75236 Query DataSets for GSE75236
Status Public on Nov 21, 2015
Title Haemophilus ducreyi transcriptome during human infection
Organism [Haemophilus] ducreyi
Experiment type Expression profiling by high throughput sequencing
Summary To better understand the molecular mechanisms underlying Haemophilus ducreyi infection in humans, here we determined the transcriptional profile of H. ducreyi in human lesions using RNA-Seq and compared it to that of in vitro growth. We were able to show that the in vivo transcriptome did not resemble that of in vitro growth. Compared to the inoculum, H. ducreyi harvested from pustules differentially expressed ~120 genes, of which 68 were upregulated. A large proportion of the upregulated genes encoded homologs of proteins involved in nutrient transport, alternative carbon pathways, growth arrest response, heat shock response, and DNA recombination. H. ducreyi upregulated few genes or operons (hgbA, flp-tad, and lspB-lspA2) required for human infection; expression of these genes is known to increase under nutrient stress. Homologs of several genes involved in anaerobic metabolism and ascorbate utilization were upregulated in vivo, suggesting that the organism is adjusting its metabolism to anaerobiosis in vivo.
Overall design RNA from Haemophilus ducreyi infected pustules were collected from four volunteers and performed RNA-Seq.
Contributor(s) Gangaiah D, Spinola SM
Citation(s) 26930707
Submission date Nov 20, 2015
Last update date Jun 28, 2019
Contact name Dharanesh Gangaiah
Phone 317-278-1027
Organization name Indiana University School of Medicine
Department Department of Microbiology and Immunology
Lab Spinola lab
Street address 635 Barnhill drive, MS 420
City Indianapolis
State/province Indiana
ZIP/Postal code 46202
Country USA
Platforms (1)
GPL21166 Illumina HiSeq 2500 ([Haemophilus] ducreyi)
Samples (4)
GSM1946558 Biopsy412
GSM1946559 Biopsy413
GSM1946560 Biopsy437
BioProject PRJNA302862
SRA SRP066474

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Supplementary file Size Download File type/resource
GSE75236_Processed_data-Biopsy.xlsx 581.9 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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