|
| Status |
Public on Nov 21, 2015 |
| Title |
Transcriptomes of 35000HP, ΔcpxA, ΔcpxR mutant strains of Haemophilus ducreyi in mid-log, transition, and stationary phase |
| Organism |
[Haemophilus] ducreyi |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To better understand the role of H. ducreyi CpxRA in controlling virulence determinants, here we defined genes potentially regulated by CpxRA by using RNA-Seq. Activation of CpxR by deletion of cpxA repressed nearly 70% of its targets, including seven established virulence determinants. Inactivation of CpxR by deletion of cpxR differentially regulated few genes and increased the expression of one virulence determinant. We identified a CpxR binding motif that was enriched in downregulated but not upregulated targets. These data reinforce the hypothesis that CpxA phosphatase activity plays a critical role in controlling H. ducreyi virulence in vivo. Characterization of the downregulated genes may offer new insights into pathogenesis.
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| |
|
| Overall design |
RNA of Haemophilus ducreyi wildtype, cpxA and cpxR mutants were collected at mid-log, transition, and stationary phases of growth, in quadruplicate.
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| |
|
| Contributor(s) |
Gangaiah D, Spinola SM |
| Citation(s) |
23729647 |
| |
| Submission date |
Nov 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Dharanesh Gangaiah |
| E-mail(s) |
dgangaia@iupui.edu
|
| Phone |
317-278-1027
|
| Organization name |
Indiana University School of Medicine
|
| Department |
Department of Microbiology and Immunology
|
| Lab |
Spinola lab
|
| Street address |
635 Barnhill drive, MS 420
|
| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21166 |
Illumina HiSeq 2500 ([Haemophilus] ducreyi) |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA302861 |
| SRA |
SRP066475 |
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