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Series GSE75237 Query DataSets for GSE75237
Status Public on Nov 21, 2015
Title Transcriptomes of 35000HP, ΔcpxA, ΔcpxR mutant strains of Haemophilus ducreyi in mid-log, transition, and stationary phase
Organism [Haemophilus] ducreyi
Experiment type Expression profiling by high throughput sequencing
Summary To better understand the role of H. ducreyi CpxRA in controlling virulence determinants, here we defined genes potentially regulated by CpxRA by using RNA-Seq. Activation of CpxR by deletion of cpxA repressed nearly 70% of its targets, including seven established virulence determinants. Inactivation of CpxR by deletion of cpxR differentially regulated few genes and increased the expression of one virulence determinant. We identified a CpxR binding motif that was enriched in downregulated but not upregulated targets. These data reinforce the hypothesis that CpxA phosphatase activity plays a critical role in controlling H. ducreyi virulence in vivo. Characterization of the downregulated genes may offer new insights into pathogenesis.
Overall design RNA of Haemophilus ducreyi wildtype, cpxA and cpxR mutants were collected at mid-log, transition, and stationary phases of growth, in quadruplicate.
Contributor(s) Gangaiah D, Spinola SM
Citation(s) 23729647
Submission date Nov 20, 2015
Last update date May 15, 2019
Contact name Dharanesh Gangaiah
Phone 317-278-1027
Organization name Indiana University School of Medicine
Department Department of Microbiology and Immunology
Lab Spinola lab
Street address 635 Barnhill drive, MS 420
City Indianapolis
State/province Indiana
ZIP/Postal code 46202
Country USA
Platforms (1)
GPL21166 Illumina HiSeq 2500 ([Haemophilus] ducreyi)
Samples (36)
GSM1946562 35000HP_midlog_rep1
GSM1946563 35000HP_midlog_rep2
GSM1946564 35000HP_midlog_rep3
BioProject PRJNA302861
SRA SRP066475

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75237_Processed_data-CpxRA.xlsx 1.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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