|
| Status |
Public on Dec 01, 2016 |
| Title |
HAL2 overexpression induces iron acquisition and enhanced salt resistance in bdf1Δ |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
The strain bdf1Δ+HAL2 improved salt resistance of bdf1∆. To gain further insight into the mechanism of bdf1∆ salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1∆ cells and the process of how HAL2 overexpression and HDA1 deletion improves salt resistance. Transcriptomic analysis under salt treatment (0.5 mol.L-1 NaCl for 45 min) was performed using four different strains: bdf1∆, W303, bdf1Δ+HAL2 and bdf1∆hda1∆. The transcription of 1,393 genes were significantly changed( > 2-fold) in bdf1∆ compared with W303 upon NaCl stress (0.5 mol.L-1 NaCl for 45 min). Only 108 genes were significantly changed ( > 2-fold) in bdf1Δ+HAL2 compared with bdf1∆ upon NaCl stress (0.5 mol.L-1 NaCl for 45 min). Only 88 genes were significantly changed ( > 2-fold) in bdf1Δ+HAL2 compared with bdf1∆ upon NaCl stress (0.5 mol.L-1 NaCl for 45 min).
|
| |
|
| Overall design |
bdf1∆ vs W303 upon NaCl stress (0.5 mol.L-1 NaCl for 45 min);bdf1Δ+HAL2 vs bdf1Δ under salt treatment (0.5 mol.L-1 NaCl for 45 min); bdf1Δhda1Δ vs bdf1Δ under salt treatment (0.5 mol.L-1 NaCl for 45 min).
|
| |
|
| Contributor(s) |
Chen L, Wang M, Hou J, Fu J, Shen Y, Fanghua L, Zhang Z, Bao X |
| Citation(s) |
27387517 |
| |
| Submission date |
Dec 09, 2015 |
| Last update date |
Mar 08, 2017 |
| Contact name |
Xiaoming Bao |
| E-mail(s) |
bxm@sdu.edu.cn
|
| Phone |
(86) 053188365826
|
| Organization name |
Shandong University
|
| Department |
State Key Laboratory of Microbial Technology
|
| Lab |
Bao lab
|
| Street address |
Shanda Nan Road 27
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250100 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA305490 |
Less...
More...