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Series GSE7609 Query DataSets for GSE7609
Status Public on Jan 03, 2008
Title Halobacterium NRC-1, EMS Stress Response
Organism Halobacterium salinarum NRC-1
Experiment type Expression profiling by array
Summary 100mM EMS was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of EMS for 30 minutes, cultures were spun down and pellets were re-suspended in same volume of GN101 media. Cultures were then harvested by centrifugation and pellets were snap frozen on a dry-ice ethanol bath. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes.
Keywords: stress response, dose response
 
Overall design 16 samples (8 samples from EMS perturbed and 8 non-perturbed controls) were analyzed on replicate arrays (dye-flips) all against the same standard reference sample.
 
Contributor(s) Kaur A, Baliga NS
Citation(s) 18160043
Submission date Apr 24, 2007
Last update date Jan 15, 2017
Contact name Marc Facciotti
E-mail(s) mfacciotti@systemsbiology.org
Organization name Institute for Systems Biology
Street address 1441 North 34th Street
City Seattle
State/province WA
ZIP/Postal code 98103
Country USA
 
Platforms (1)
GPL3739 ISB Halobacterium Spotted Microarray
Samples (32)
GSM182477 Halobacterium EMS exposure experiment, Array 8162
GSM182478 Halobacterium EMS exposure experiment, Array 8163
GSM182479 Halobacterium EMS exposure experiment, Array 8164
Relations
BioProject PRJNA100173

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7609_RAW.tar 38.1 Mb (http)(custom) TAR (of CSV)

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