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| Status |
Public on Dec 28, 2016 |
| Title |
Trithorax-group protein ASH1 defines novel H3K36me3/H3K9me3 bivalent domains |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
A Trithorax-group (TrxG) protein ASH1 selectively dimethylates histone H3 lysine 36 (H3K36) and is required for expression of Hox genes. Genome-wide targets and molecular functions of ASH1, however, have remained elusive. Here we report ChIP-seq analysis of ASH1 targets in the human genome using a leukemia cell line K562. We identified 4,596 ASH1 binding sites, 2,502 of which are in the coding region, mostly in a proximity of promoters. Remaining half of the ASH1 binding sites are in the intergenic region. Analysis of histone methyation patterns revealed that a majority of ASH1 bidning sites are closely associated not only with trimethylated H3K36 (H3K36me3) but also with H3K9me3, shedding light for the first time to H3K36/H3K9 double methylation domains. Of note, an H3K9 methyltransferase SETDB1 is enriched in the ASH1 bidning sites, whereas EZH2 but not SUZ12 of the Polycomb Repression Complex 2 (PRC2) is present in bivalent domains. In addition, a small subset of ASH1 targets bear H3K4me3 and colocalize with RNA polymerase II. These select targets are highly enriched for regulators of gene expression, such as CXXC1, WHSC2/NELFA, and a battery of zinc finger proteins, suggesting that ASH1 might acts as a master regulator of gene expression.
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| Overall design |
Two biological replicates using different batches of affinity purified rabbit polyclonal antibodies against the C-terminal 18 amino acids of human/mouse ASH1.
Anti-ASH1 antibodies were made in-house: batch #1 (Tanaka et al. Gene 397:161-168, 2007) and batch #3.
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| Contributor(s) |
Tanaka Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 18, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yujiro Tanaka |
| E-mail(s) |
yujirotanaka@gmail.com
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| Organization name |
Tokyo Medical and Dental University
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| Department |
School of Biomedical Science
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| Lab |
Genome Structure and Regulation
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| Street address |
2-3-10 Kandasurugadai
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| City |
Chiyodaku |
| State/province |
Tokyo |
| ZIP/Postal code |
101-0062 |
| Country |
Japan |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA306482 |
| SRA |
SRP067569 |
