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Series GSE76461 Query DataSets for GSE76461
Status Public on Jun 12, 2017
Title The role of Sgf73 occupancy
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Sgf73, a core component of the SAGA (Spt-Ada-Gcn5 Acetyltransferase) co-activator complex, is the yeast orthologue of ataxin-7, which in humans undergoes CAG ? polyglutamine repeat expansion to produce the neurodegenerative disease spinocerebellar ataxia type 7 (SCA7). We recently documented that deletion of SGF73 dramatically extends replicative lifespan (RLS) in yeast. To define the basis for Sgf73-mediated RLS extension, and to further explore the molecular function of Sgf73/ataxin-7 we performed ChIP-Seq for Sgf73 in yeast to identify its regions of DNA binding. The aim of the study was to uncover the underlying mechanisms controlled by Sgf73. This information will be used to understand the role of Sgf73 target genes in yeast aging pathways and possibly highlight pathways of mammalian aging regulation and SCA7 neurodegeneration in humans.
Overall design Examination of Sgf73 occupancy in wildtype S. cerevisiae
Web link;jsessionid=72C8B7B277DB98289BB038D59120160B.f02t02
Contributor(s) Mason AG, Garza RM, McCormick MA, Kennedy BK, Pillus L, La Spada AR
Citation(s) 28568901
Submission date Dec 31, 2015
Last update date May 15, 2019
Contact name Albert R. La Spada
Organization name University of California San Diego
Department Pediatrics
Street address 2880 Torrey Pines Scenic Dr.
City La Jolla
State/province California
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL13821 Illumina HiSeq 2000 (Saccharomyces cerevisiae)
Samples (5)
GSM2025015 WT ChIPseq_1
GSM2025016 WT ChIPseq_2
GSM2025017 Sgf73-Myc ChIPseq_1
BioProject PRJNA307564
SRA SRP068020

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Supplementary file Size Download File type/resource
GSE76461_RAW.tar 32.0 Mb (http)(custom) TAR (of BED, TXT)
GSE76461_WTMycChIP.renamed.bed.gz 2.7 Mb (ftp)(http) BED
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Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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