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Series GSE76686 Query DataSets for GSE76686
Status Public on Jan 09, 2016
Title Engineered stomach tissue as a renewable source of functional beta-cells for blood glucose regulation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The gastrointestinal (GI) epithelium is a highly regenerative tissue with the potential to provide a renewable source of insulin+ cells using cellular reprogramming. Here, we describe the antral stomach as a previously unrecognized source highly amenable to conversion into functional insulin-secreting cells. Native antral endocrine cells share a surprising degree of transcriptional similarity with pancreatic beta-cells. Expression of beta-cell reprogramming factors in vivo converts antral cells efficiently into insulin+ cells with close molecular and functional resemblance to beta-cells. Our data further indicate that the intestine-expressed Cdx2 acts as a molecular barrier for beta-cell conversion. Induced GI insulin+ cells can suppress hyperglycemia over at least 6 months and they regenerate rapidly after ablation from the native stem-cell compartment. Transplantation of bioengineered stomach mini-organs also produced insulin+ cells and suppressed hyperglycemia. These studies demonstrate the potential of developing engineered stomach tissue as a renewable source of functional beta-cells for glycemic control.
Overall design Total RNA extracted from primary mouse tissues: Stomach (3 replicates), Duodenum (3 replicates) and Colon (2 replicates)
Contributor(s) Ariyachet C, Tovaglieri A, Xiang G, Lu J, Shah MS, Richmond CA, Verbeke C, Brown J, Shivdasani RA, Mahony S, Mooney D, Melton DA, Xia Q, Breault DT, Zhou Q
Citation(s) 26908146
Submission date Jan 08, 2016
Last update date Feb 27, 2016
Contact name Shaun Mahony
Phone 814-865-3008
Organization name Penn State University
Department Biochemistry & Molecular Biology
Lab Shaun Mahony
Street address 404 South Frear Bldg
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
Platforms (1)
GPL15097 Illumina MouseRef-8 v2.0 expression beadchip (ILMN_GENE)
Samples (8)
GSM2035732 gastric enteroendocrine cells 1
GSM2035733 gastric enteroendocrine cells 2
GSM2035734 gastric enteroendocrine cells 3
BioProject PRJNA308316

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Supplementary file Size Download File type/resource
GSE76686_RAW.tar 9.4 Mb (http)(custom) TAR (of IDAT)
Processed data included within Sample table

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