GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Series GSE773

Query DataSets for GSE773

Status

Public on Nov 25, 2003

Title

Thyroid RDC8 versus C57Bl6

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Mutations of the thyrotropin receptor leading to constitutive activation of the cAMP cascade are responsible for the development of hot nodules, if arising in a somatic cell, and non-auto-immune hyperthyroidism, when occurring in a germinal cell. An animal model of constitutive activation of the thyroid cAMP cascade has been obtained by generating transgenic mice expressing the adenosine receptor (Tg-A2aR) under the control of the thyroglobulin promoter. These mice develop huge goiters and die prematurely due to hyperthyroidism induced cardiac failure. To identify new genes involved in the tumorigenic pathway of the thyroid, we designed a protocol using microarray technology to study the differential expression, between normal and transgenic thyroid, of +/- 13.000 genes. 360 genes or EST showed a strong modulation with background corrected values of fluorescence superior to 2 fold change. The modulated genes were classified according to their proposed gene ontology functions. Approximately half of them were upregulated. The function of the majority of these genes in thyroid physiology is still to be determined. Some of them, like IGF-I, IGF-BP3, IGF-BP5, may play an important role in the development of thyroid nodules through paracrine mechanisms.
Keywords = Thyroid
Keywords = Adenosine Receptor 2a
Keywords: parallel sample

Web link

http://www.microarrays.be

Contributor(s)

Goffard J, Jin L, Mirescu H, Van Hummelen P, Ledent C, Dumont J, Corvilain B

Citation(s)

14563936

Submission date

Oct 23, 2003

Last update date

Jan 18, 2013

Contact name

Rekin's Janky