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Series GSE77363 Query DataSets for GSE77363
Status Public on Apr 25, 2016
Title Genome-wide binding pattern of β-catenin during Xenopus gastrulation
Organism Xenopus laevis
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach.
Overall design Xenopus laevis emrbyos were injected with a triple FLAG tagged beta-catenin at levels that did not effect phenotype. Pools of about 500 embryos were fixed for ChIP processing. An input sample was taken prior to immunoprecipitation with a Flag antibody. Both input and ChIP material were used to make sequencing libraries.
Contributor(s) Kjolby RS, Harland RM
Citation(s) 27091726
Submission date Jan 28, 2016
Last update date May 15, 2019
Contact name Richard Harland
Organization name University of California, Berkeley
Street address Life Sciences Addition #3200
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
Platforms (1)
GPL17682 Illumina HiSeq 2000 (Xenopus laevis)
Samples (6)
GSM2050738 ChIP-beta-catenin-2
GSM2050739 ChIP-beta-catenin-6
GSM2050740 ChIP-beta-catenin-19
This SubSeries is part of SuperSeries:
GSE77365 Wnt/β-catenin in Xenopus laevis gastrulation
BioProject PRJNA310141
SRA SRP069034

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77363_RAW.tar 1.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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