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Series GSE78147 Query DataSets for GSE78147
Status Public on Mar 07, 2018
Title Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type Young and Old muscle Transcriptomes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare Young and Old muscles transcriptome profiling (RNA-seq) to optimal high-throughput data analysis
Results: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the mouse genome (build mm10) and identified 24799 genes. Among them, 95.7% of the genes displayed the similar expression level in both young and old muscles.2% of genes (501) were significantly up-regulated and 2.3% of genes (584) were significantly down-regulated. p value <0.05.
Overall design Muscle mRNA profiles of 3-month old wild type (WT young) and 20-month old wild type mice (WT Old) were generated by deep sequencing, in triplicate, using Illumina HiSeq 3000.
Contributor(s) Hu P, Yin J, Xu B, Xue L
Citation(s) 29717119
Submission date Feb 22, 2016
Last update date May 22, 2019
Contact name Jie Yin
Organization name Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Street address 320 Yueyang Road
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM2068000 Old1
GSM2068001 Old2
GSM2068002 Young1
BioProject PRJNA312777
SRA SRP070644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78147_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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