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Status |
Public on Oct 31, 2003 |
Title |
Corticosteroid responsive genes |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by SAGE
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Summary |
Three groups of Wistar rats with different manipulated levels of corticosterone were used to identify corticosteroid-responsive genes in hippocampus: 1. rats were adrenalectomised (ADX) 2. In addition to ADX these rats were implanted with a subcutaneous pellet containing 20 mg corticosterone and 80% cholesterol (ADX + low CORT) 3. In addition to ADX and implantation of a corticosterone pellet, these rats received a subcutaneous injection with corticosterone (1 mg/kg bodyweight) three hours prior to decapitation ((ADX + low CORT) + acute high CORT).
All 3 groups of rats were decapitated three days after ADX and their hippocampus was isolated. Hippocampal RNA from 6 rats per group was pooled and used as input material for the SAGE libraries. Keywords: parallel sample
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Contributor(s) |
Datson N, van der Perk J, de Kloet E, Vreugdenhil E |
Citation(s) |
11556892 |
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Submission date |
Oct 30, 2003 |
Last update date |
Mar 02, 2012 |
Contact name |
Nicole Datson |
E-mail(s) |
datson_n@lacdr.leidenuniv.nl
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Phone |
(+31)71-5276222
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Organization name |
Leiden University
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Department |
Medical Pharmacology
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Street address |
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City |
Leiden |
ZIP/Postal code |
2333 CC |
Country |
Netherlands |
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Platforms (1) |
GPL23 |
SAGE:10:NlaIII:Rattus norvegicus |
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Samples (3) |
GSM12542 |
rat hippocampus: adrenalectomised rat (ADX) |
GSM12543 |
rat hipocampus: ADX + low CORT |
GSM12544 |
rat hippocampus: (ADX + low CORT)+ acute high CORT |
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Relations |
BioProject |
PRJNA87755 |