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Series GSE7878 Query DataSets for GSE7878
Status Public on Aug 01, 2007
Title Integrated genome-wide analysis of gene dosage and gene expression in Non Small Cell Lung Cancer
Organism Homo sapiens
Experiment type Genome variation profiling by array
Summary Understanding the molecular events in non-small cell lung cancer (NSCLC) is essential to improve early diagnosis and treatment for this disease. We examined the effect of chromosomal copy number changes on gene expression in resected NSCLC patients. We identified a deletion on 14q32.2-33 as a common alteration in NSCLC (44%), which significantly influenced gene expression for HSP90AA1, residing on 14q32. This deletion was correlated with better overall and recurrence free survival (P=0.008 and P=0.004, respectively) and survival was also longer in patients whose tumors had low expression levels of HSP90AA1. We extended the analysis to an independent validation set of 140 resected NSCLC patients, and confirmed low HSP90AA1 expression to be significantly related with overall survival and recurrence free survival (P=0.003 and P=0.007, respectively). In vitro treatment with an HSP90 inhibitor had potent antiproliferative activity in NSCLC cell lines. We suggest that targeting HSP90 will have clinical impact for NSCLC patients.
Keywords: Array CGH gene expression integration
Overall design Array CGH and gene expression arrays (data available via array express accession: 0000) were performed on 32 radically resected tumor samples from stage I and II NSCLC patients. An integrative analysis tool ACE-it was applied to determine whether chromosomal copy number affects gene expression.
Contributor(s) GallegosRuiz MI, Floor K, Roepman P, Rodriguez JA, Meijer GA, Mooi WJ, Jassem E, Niklinski J, Muley T, van Zandwijk N, Smit EF, Beebe K, Neckers L, Ylstra B, Giaccone G
Citation(s) 18320023
Submission date May 23, 2007
Last update date Mar 16, 2012
Contact name Daoud Sie
Phone +31 20 4442428
Organization name Vrije Universiteit Medical Center
Department Pathology
Lab Microarray Core Facility
Street address De Boelelaan 1117
City Amsterdam
ZIP/Postal code 1081 HV
Country Netherlands
Platforms (1)
GPL2827 VUMC MACF human 30K oligo v44
Samples (32)
GSM190616 Stage T1N0 NSCLC 0464832
GSM190633 Stage T2N0 NSCLC 3977909
GSM190634 Stage T1N0 NSCLC 3460541
BioProject PRJNA99907

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Supplementary file Size Download File type/resource
GSE7878_RAW.tar 95.0 Mb (http)(custom) TAR (of XLS)
Processed data included within Sample table

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