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Series GSE7906 Query DataSets for GSE7906
Status Public on Sep 01, 2009
Title Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens
Organisms Mus musculus; Rattus norvegicus
Experiment type Expression profiling by array
Summary SV40 transforms cells through the action of two oncoproteins, large T antigen and small t antigen. Small t antigen targets phosphatase PP2A, while large T antigen stimulates cell proliferation and survival by action on multiple proteins, including the tumor suppressors Rb and p53. Large T antigen also binds components of the transcription initiation complex and several transcription factors. We examined global gene expression in SV40-transformed mouse embryo fibroblasts, and in enterocytes obtained from transgenic mice. SV40 transformation alters the expression of approximately 800 cellular genes in both systems. Much of this regulation is observed in both MEFs and enterocytes and is consistent with T antigen action on the Rb-E2F pathway. However, the regulation of many genes is cell-type specific, suggesting that unique signaling pathways are activated in different cell types upon transformation, and that the consequences of SV40 transformation depends on the type of cell targeted.
Overall design We used the Affymetrix RGU34A microarrays to probe the global mRNA expression from two wild-type REF52neo and three REF52neoT stable cell lines. For the MEF microarrays, we used a dye-flip reference design (Churchill GA, 2002, Nature Genetics, Vol. 32, p.490-495) to compare a pool of MEF total RNA to three independent T antigen MEF cell lines. All REF52 and MEF cells were grown to two days post-confluence and harvested. For the mouse small intestine microarrays, we used three different intestinal preparations: Whole, Villi and Vlcm in a replicated dye-flip design (Churchill GA) using three (Vlcm) or four (Whole and Villi) T+ mice and the same number of their wild-type littermates for each intestinal preparation. We used the Agilent Mouse cDNA (G4104A) array for the MEF and small intestine experiments.
Contributor(s) Cantalupo PG
Citation(s) 19201438
Submission date May 25, 2007
Last update date Feb 21, 2017
Contact name Daniel Clark
Organization name University of Pittsburgh
Street address 3501 Terrace St.
City Pittsburgh
ZIP/Postal code 15213
Country USA
Platforms (3)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
GPL871 Agilent Mouse cDNA Microarray (G4104A) [layout A]
GPL872 Agilent Mouse cDNA Microarray (G4104A) [layout B]
Samples (35)
GSM190145 REF52neo, bio rep 1
GSM190146 REF52neo, bio rep 2
GSM190147 REF52neoTAg, bio rep 1
BioProject PRJNA100081

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7906_RAW.tar 102.4 Mb (http)(custom) TAR (of CEL, TSV)
GSE7906_Readme.txt 2.9 Kb (ftp)(http) TXT
Processed data included within Sample table

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