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| Status |
Public on Dec 06, 2016 |
| Title |
Chromatin immunoprecipitation and sequencing of pPLT2::PLT2-YFP in the root of plt1plt2 double mutant |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Organ formation in animals and plants relies on precise control of cell state transitions to turn stem cell daughters into fully differentiated cells. In plants, cells cannot rearrange due to shared cell walls. Thus, differentiation progression and the accompanying cell expansion must be tightly coordinated. PLETHORA (PLT) transcription factor gradients were shown to guide the progression of cell differentiation at different positions in the Arabidopsis root. While well-described transcription factor gradients in animals specify distinct cell fates within an essentially static context, the PLT gradient is unique in its ability to control cell differentiation in a growing organ during continuous production and expansion of cells. To understand the output of these gradients we studied the gene set transcriptionally controlled by PLTs. Our work reveals how the PLT gradient regulates cell state by region-specific induction of cell proliferation genes and repression of differentiation. Moreover, PLT targets include major patterning genes and autoregulatory feedback components, reinforcing their role as master regulators of organ development.
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| Overall design |
Two replicates were performed for the IP and sequenced along with the Input DNA.
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| Contributor(s) |
Santuari L |
| Citation(s) |
27920338 |
| |
| Submission date |
Mar 31, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Luca Santuari |
| Organization name |
Wageningen University
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| Department |
Plant Sciences Group
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| Lab |
Plant Developmental Biology
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| Street address |
Droevendaalsesteeg 1
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| City |
Wageningen |
| ZIP/Postal code |
6708PB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL10088 |
AB SOLiD System 3.0 (Arabidopsis thaliana) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE79755 |
The PLETHORA gene regulatory network guides growth and cell differentiation in Arabidopsis roots |
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| Relations |
| BioProject |
PRJNA316934 |
| SRA |
SRP072664 |
