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Series GSE8009 Query DataSets for GSE8009
Status Public on Dec 31, 2012
Title NF-kB target genes in dendritic cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary The ability of dendritic cells (DC) to initiate immunity and induce antigen-specific tolerance makes DC ideal targets for pharmacological intervention into immune responses. NF-kB factors are a family of transcriptional regulators important for DC development and function. However, the identity of NF-kB target genes that are central to DC biology is largely unknown. In the present study IkBa super repressor (IkBa-SR) and DNA microarray analysis were used to determine the repertoire of NF-kB responsive genes in DC. In DC these genes regulate vital DC functions of antigen uptake and presentation, motility, survival, etc.

Taking in account limitations of the genome-wide microarray analysis, generated transcription factor data were confirmed by the independent means of RT-PCR and chromatin immunoprecipitation. Kinetics of NF-kB induction by well-known DC activatory agents TNFa and LPS were further analysed. NF-kB regulated genes can be potentially useful targets for the development of more specific anti-inflammatory agents for clinical applications.
Keywords: drug treatment, adenovirus transduction
 
Overall design DC were generated in vitro from bone marrow of CAR transgenic mice as described previously (Hieronymus, T., T. C. Gust, R. D. Kirsch, T. Jorgas, G. Blendinger, M. Goncharenko, K. Supplitt, S. Rose-John, A. M. Muller, and M. Zenke. 2005. Progressive and controlled development of mouse dendritic cells from Flt3+CD11b+ progenitors in vitro. J Immunol 174:2552-2562). Immature DC were transduced with AdIkBa-SR adenovirus, AdOVA control virus, or left untreated. Cells were then stimulated with TNFa, or left unstimulated. Total RNA was amplified, labelled and hybridised to Affymetrix MOE430A arrays. TNFa up- and down-regulated genes, as well as genes regulated by AdOVA control virus, and suppressed by AdIkBa-SR were analysed.
 
Contributor(s) Goncharenko M, Kaergel E, Hieronymus T, Gust T, Cotton M, Scheidereit C, Zenke M
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Submission date Jun 04, 2007
Last update date Jan 08, 2019
Contact name Mykola Goncharenko
E-mail(s) nick.goncharenko@berlin.de
Phone +492418080763
Fax +492418082442
URL http://molcell.de
Organization name IBMT - Cell Biology, University Hospital, RWTH Aachen
Department Institute for Biomedical Technology - Cell Biology
Street address Pauwelstrasse 30
City Aachen
ZIP/Postal code 52074
Country Germany
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (6)
GSM197702 2-step DC - untreated
GSM197705 2-step DC - TNFa
GSM197707 2-step DC - AdOVA
Relations
BioProject PRJNA100801

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8009_RAW.tar 55.9 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data included within Sample table
Processed data provided as supplementary file

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