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Series GSE8011 Query DataSets for GSE8011
Status Public on Aug 14, 2007
Title Methylation of hMTase G9a on a histone-like site regulates protein complex assembly
Organism Mus musculus
Experiment type Expression profiling by array
Methylation profiling by array
Summary Gene expression in eukaryotes is tightly linked to the methylation state of specific lysine residues within the N-terminal region of the core histone proteins. While the mechanisms connecting histone lysine methylation to effector protein recruitment and control of gene activity are increasingly well understood, it remains unknown whether non-histone chromatin proteins are targets for similar modification-recognition systems. Here we show that histone H3 and the H3 methyltransferase G9a share a conserved methylation motif that is both necessary and sufficient to mediate in vivo interaction with the potent epigenetic regulator Heterochromatin Protein 1 (HP1). As with H3, G9a-HP1 interaction is dependent on lysine methylation and can be reversed by adjacent phosphorylation. NMR analysis demonstrates that the HP1 chromodomain recognizes methyl-G9a through a binding mode similar to that used in recognition of methyl-H3, and that adjacent phosphorylation directly antagonizes G9a-HP1 interaction. In addition to uncovering the chromodomain as a generalized methyl-lysine binding module, these data identify histone-like modification cassettes (or “histone mimics”) as an entirely new class of non-histone methylation targets, and directly demonstrate the relevance of the principles underlying the histone code to the regulation of non-histone proteins.
Keywords: methylation analysis
Overall design Two independent Affymetrix gene expression microarray analyses were performed on samples from G9a-deleted MEFs reconstituted with empty vector (delta), wild type FLAG-G9a (WT), FLAG-G9a K165A (K165A) or FLAG-G9a H1093K catalytic mutant (H1093K).
Contributor(s) Sampath SC, Tarakhovsky A
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Submission date Jun 04, 2007
Last update date May 04, 2018
Contact name Agnes Viale
Phone 212 639 3031
Fax 212 717 3053
Organization name Memorial Sloan Kettering Cancer Center
Department Molecular Biology
Lab Genomics Core Laboratory
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (10)
GSM197740 G9a-WT
GSM197741 G9a-WT_2
GSM197744 G9a-delta
BioProject PRJNA100805

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Supplementary file Size Download File type/resource
GSE8011_RAW.tar 91.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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