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| Status |
Public on Oct 28, 2016 |
| Title |
Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Other
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| Summary |
RNA-DNA hybrids are a major internal cause of DNA damage within cells, and their degradation by RNAse H enzymes is important for maintaining genomic stability.
Here, we identified an unexpected role for RNA-DNA hybrids and RNase H enzymes in DNA repair.
Using a site-specific DNA double-stranded break (DSB) system in Schizosaccharomyces pombe, we showed that RNA-DNA hybrids form as part of the homologous recombination (HR)-mediated DSB repair process
and RNase H enzymes are essential for their degradation and efficient completion of DNA repair.
Deleting RNase H stabilizes RNA-DNA hybrids around DSB sites and strongly impairs recruitment of the ssDNA-binding RPA complex.
In contrast, overexpressing RNase H1 destabilizes these hybrids, leading to excessive strand resection and RPA recruitment, and to severe loss of repeat regions around DSBs.
Our study challenges the existing model of HR-mediated DSB repair, and reveals a surprising role for RNA-DNA hybrids in maintaining genomic stability.
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| Overall design |
Inducible Double Strand Break (DSB) system. 6 samples, all in duplicates (12 samples alltogether)
Insert sequence file (DSB.system.insert.txt); modified S. pombe genome sequence (Schizosaccharomyces_pombe.ASM294v2.29.dna.genome.modified_DSBsystem03032016.fa), and modified S. pombe annotation file (Schizosaccharomyces_pombe.ASM294v2.29.dna.genome.modified_DSBsystem03032016.bed)
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| Contributor(s) |
Ohle C, Tesorero R, Schermann G, Dobrev N, Sinning I, Fischer T |
| Citation(s) |
27881299 |
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| Submission date |
Apr 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Tamas Fischer |
| E-mail(s) |
tamas.fischer@bzh.uni-heidelberg.de
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| Phone |
+49 6221 544728
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| Organization name |
Biochemie Zentrum Heidelebrg
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| Department |
BZH
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| Lab |
AG Fischer
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| Street address |
Im Neuenheimer Feld 328
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
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| Samples (12)
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| GSM2125959 |
RNA-DNA hybrid ChIP-exo, Induced, Rep1 |
| GSM2125960 |
RNA-DNA hybrid ChIP-exo, Uninduced, Rep1 |
| GSM2125961 |
RNA-DNA hybrid ChIP-exo, Induced, Rep2 |
| GSM2125962 |
RNA-DNA hybrid ChIP-exo, Uninduced, Rep2 |
| GSM2125963 |
Ssb2-Flag ChIP-exo, Rnh1 overexp. , Induced, Rep1 |
| GSM2125964 |
Ssb2 ChIP-exo, WT, Uninduced, Rep1 |
| GSM2125965 |
Ssb2 ChIP-exo, WT, Induced, Rep1 |
| GSM2125966 |
Ssb2 ChIP-exo, Rnh1del-Rnh201del, Induced, Rep1 |
| GSM2125967 |
Ssb2 ChIP-exo, Rnh1 overexp., Induced, Rep2 |
| GSM2125968 |
Ssb2 ChIP-exo, WT, Uninduced, Rep2 |
| GSM2125969 |
Ssb2 ChIP-exo, WT, Induced, Rep2 |
| GSM2125970 |
Ssb2 ChIP-exo, Rnh1del-Rnh201del, Induced, Rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE84883 |
Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair |
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| Relations |
| BioProject |
PRJNA318794 |
| SRA |
SRP073455 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE80398_DSB.system.insert.txt.gz |
645 b |
(ftp)(http) |
TXT |
| GSE80398_RAW.tar |
1.9 Gb |
(http)(custom) |
TAR (of BW) |
| GSE80398_Schizosaccharomyces_pombe.ASM294v2.29.dna.genome.modified_DSBsystem03032016.bed.gz |
78.7 Kb |
(ftp)(http) |
BED |
| GSE80398_Schizosaccharomyces_pombe.ASM294v2.29.dna.genome.modified_DSBsystem03032016.fa.gz |
3.5 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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