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| Status |
Public on Dec 19, 2017 |
| Title |
Single Cell DNA Methylome Sequencing of Human Preimplantation Embryos |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Genome variation profiling by high throughput sequencing
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| Summary |
Although the DNA methylome of human early embryos has been analyzed, some of the key features have not been addressed to date. Here, we performed single-cell DNA methylome sequencing for human preimplantation embryos and found that tens of thousands of genomic loci exhibited de novo DNA methylation. This finding indicates that genome-wide DNA methylation reprogramming during preimplantation development is a dynamic balance between strong global demethylation and significant focused re-methylation. Furthermore, the demethylation of the paternal genome is much faster and thorough than that of the maternal genome. From the 2-cell to post-implantation stage, methylation of the paternal genome is consistently lower than that on the maternal genome. We also showed that the genetic lineage of the early blastomeres could be traced by DNA methylation analysis. Our work paves the way for deciphering the secrets of DNA methylation reprogramming in human early embryos.
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| Overall design |
We did single cell PBAT DNA methylome sequencing analysis for cells from human mature oocytes and preimplantation embryos. For each individual cell, we sequenced 8.4 Gb of data on average.
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| Contributor(s) |
Zhu P, Guo H, Ren Y, Hou Y, Yan L, Qiao J, Tang F |
| Citation(s) |
29255258 |
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| Submission date |
May 09, 2016 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hongshan Guo |
| E-mail(s) |
guohs@zju.edu.cn
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| Organization name |
Liangzhu Laboratory
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| Department |
School of Medicine, Zhejiang University
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| Street address |
No.1369, Wenyixi Road
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| City |
Hangzhou |
| State/province |
Zhejiang Province |
| ZIP/Postal code |
310000 |
| Country |
China |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (673)
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| Relations |
| BioProject |
PRJNA321024 |
| SRA |
SRP074598 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81233_RAW.tar |
404.9 Gb |
(http)(custom) |
TAR (of BED, FPKM_TRACKING, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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