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Series GSE81287 Query DataSets for GSE81287
Status Public on Oct 17, 2016
Title Small-RNA transcriptome sequencing of individual cells
Organisms Homo sapiens; synthetic construct
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Several classes of small RNAs have been described, however to molecularly profile them require large numbers of cells. Here, we developed a method for single-cell small-RNA sequencing that we applied to naïve and primed human embryonic stem cells and to cancer cells. Single-cell profiling of microRNAs and fragments of tRNAs and snoRNAs revealed that, in particular, microRNAs have unrecognized potential to separate cell types and states.
 
Overall design Small-RNA transcriptome sequencing (Small-seq) is a method designed to capture all small RNAs at single cells
 
Contributor(s) Abdullayev I, Faridani OR, Sandberg R
Citation(s) 27798564
Submission date May 10, 2016
Last update date May 15, 2019
Contact name Ilgar Abdullayev
E-mail(s) ilgar.abdullayev@licr.ki.se
Organization name Karolinska Institutet
Department Cell and Molecular Biology
Lab Rickard Sandberg's group (rickard.sandberg@ki.se)
Street address Nobels väg 3
City Stockholm
ZIP/Postal code 17177
Country Sweden
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL15228 Illumina HiSeq 2000 (synthetic construct)
Samples (539)
GSM2149190 primedcell_10
GSM2149191 primedcell_11
GSM2149192 primedcell_12
Relations
BioProject PRJNA321192
SRA SRP074776

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81287_51bp_mature-smallrna_counts_molc.txt.gz 3.6 Mb (ftp)(http) TXT
GSE81287_51bp_precursor_counts_molc.txt.gz 2.1 Mb (ftp)(http) TXT
GSE81287_NEBNext_mirna_rpms.txt.gz 31.7 Kb (ftp)(http) TXT
GSE81287_allcells_mirna_postqc.txt.gz 102.0 Kb (ftp)(http) TXT
GSE81287_dilution_mirna_counts_molc.txt.gz 35.0 Kb (ftp)(http) TXT
GSE81287_smartseq2_refseq_rpkms.txt.gz 4.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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