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| Status |
Public on Jun 21, 2007 |
| Title |
Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Seedling data |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray analysis of gene expression patterns in immature ear, seedling, and embryo tissues from the maize inbred lines B73 and Mo17 identified numerous genes with variable expression. Some genes had detectable expression in only one of the two inbreds; most of these genes were detected in the genomic DNA of both inbreds, indicating that the expression differences are likely caused by differential regulation rather than by differences in gene content. Gene expression was also monitored in the reciprocal F1 hybrids B73xMo17 and Mo17xB73. The reciprocal F1 hybrid lines did not display parental effects on gene expression levels. Approximately 80% of the differentially expressed genes displayed additive expression patterns in the hybrids relative to the inbred parents. The approximately 20% of genes that display nonadditive expression patterns tend to be expressed at levels within the parental range, with minimal evidence for novel expression levels greater than the high parent or less than the low parent. Analysis of allele-specific expression patterns in the hybrid suggested that intraspecific variation in gene expression levels is largely attributable to cis-regulatory variation in maize. Collectively, our data suggest that allelic cis-regulatory variation between B73 and Mo17 dictates maintenance of inbred allelic expression levels in the F1 hybrid, resulting in additive expression patterns.
Keywords: genotype comparison
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| Overall design |
Affymetrix expression profiling was used to study gene expression in aerial tissue from 11-day seedlings of maize. Three biological replicates were performed for four different genotypes; B73, Mo17, B73xMo17 and Mo17xB73.
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| Contributor(s) |
Robert SM, Springer NM |
| Citation(s) |
16702414 |
| |
| Submission date |
Jun 19, 2007 |
| Last update date |
Apr 23, 2013 |
| Contact name |
Nathan M Springer |
| E-mail(s) |
springer@umn.edu
|
| Phone |
6126246241
|
| Fax |
6126251738
|
| Organization name |
University of Minnesota
|
| Department |
Plant Biology
|
| Street address |
1445 Gortner Ave
|
| City |
Saint Paul |
| State/province |
MN |
| ZIP/Postal code |
55108 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4032 |
[Maize] Affymetrix Maize Genome Array |
|
| Samples (12)
|
| GSM202277 |
Mo17 11-day seedling aerial tissue biological replicate 1 |
| GSM202278 |
B73 11-day seedling aerial tissue biological replicate 1 |
| GSM202279 |
Mo17xB73 11-day seedling aerial tissue biological replicate 1 |
| GSM202280 |
B73xMo17 11-day seedling aerial tissue biological replicate 1 |
| GSM202281 |
Mo17 11-day seedling aerial tissue biological replicate 2 |
| GSM202282 |
B73 11-day seedling aerial tissue biological replicate 2 |
| GSM202283 |
Mo17xB73 11-day seedling aerial tissue biological replicate 2 |
| GSM202284 |
B73xMo17 11-day seedling aerial tissue biological replicate 2 |
| GSM202285 |
Mo17 11-day seedling aerial tissue biological replicate 3 |
| GSM202286 |
B73 11-day seedling aerial tissue biological replicate 3 |
| GSM202287 |
Mo17xB73 11-day seedling aerial tissue biological replicate 3 |
| GSM202288 |
B73xMo17 11-day seedling aerial tissue biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE8194 |
Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression |
|
| Relations |
| BioProject |
PRJNA105417 |
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