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Series GSE8174 Query DataSets for GSE8174
Status Public on Jun 21, 2007
Title Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Seedling data
Organism Zea mays
Experiment type Expression profiling by array
Summary Microarray analysis of gene expression patterns in immature ear, seedling, and embryo tissues from the maize inbred lines B73 and Mo17 identified numerous genes with variable expression. Some genes had detectable expression in only one of the two inbreds; most of these genes were detected in the genomic DNA of both inbreds, indicating that the expression differences are likely caused by differential regulation rather than by differences in gene content. Gene expression was also monitored in the reciprocal F1 hybrids B73xMo17 and Mo17xB73. The reciprocal F1 hybrid lines did not display parental effects on gene expression levels. Approximately 80% of the differentially expressed genes displayed additive expression patterns in the hybrids relative to the inbred parents. The approximately 20% of genes that display nonadditive expression patterns tend to be expressed at levels within the parental range, with minimal evidence for novel expression levels greater than the high parent or less than the low parent. Analysis of allele-specific expression patterns in the hybrid suggested that intraspecific variation in gene expression levels is largely attributable to cis-regulatory variation in maize. Collectively, our data suggest that allelic cis-regulatory variation between B73 and Mo17 dictates maintenance of inbred allelic expression levels in the F1 hybrid, resulting in additive expression patterns.
Keywords: genotype comparison
Overall design Affymetrix expression profiling was used to study gene expression in aerial tissue from 11-day seedlings of maize. Three biological replicates were performed for four different genotypes; B73, Mo17, B73xMo17 and Mo17xB73.
Contributor(s) Robert SM, Springer NM
Citation(s) 16702414
Submission date Jun 19, 2007
Last update date Apr 23, 2013
Contact name Nathan M Springer
Phone 6126246241
Fax 6126251738
Organization name University of Minnesota
Department Plant Biology
Street address 1445 Gortner Ave
City Saint Paul
State/province MN
ZIP/Postal code 55108
Country USA
Platforms (1)
GPL4032 [Maize] Affymetrix Maize Genome Array
Samples (12)
GSM202277 Mo17 11-day seedling aerial tissue biological replicate 1
GSM202278 B73 11-day seedling aerial tissue biological replicate 1
GSM202279 Mo17xB73 11-day seedling aerial tissue biological replicate 1
This SubSeries is part of SuperSeries:
GSE8194 Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression
BioProject PRJNA105417

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Supplementary file Size Download File type/resource
GSE8174_RAW.tar 109.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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