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Series GSE82017 Query DataSets for GSE82017
Status Public on Apr 20, 2017
Title BRG1 Chromatin Remodeling ATPase Balances Germ Layer Patterning by Amplifying the Transcriptional Burst at Midblastula Transition [BRG1]
Organism Xenopus tropicalis
Experiment type Expression profiling by array
Summary Zygotic gene expression programs control cell differentiation in vertebrate development. In Xenopus, these programs are initiated by local induction of regulatory genes through maternal signaling activities in the wake of zygotic genome activation (ZGA) at the midblastula transition (MBT). These programs lay down the vertebrate body plan through gastrulation and neurulation, and are accompanied by massive changes in chromatin structure, which increasingly constrain cellular plasticity. Here we report on developmental functions for Brahma related gene 1 (BRG1), a key component of embryonic SWI/SNF chromatin remodeling complexes. Carefully controlled, global BRG1 protein depletion in X. tropicalis and X. laevis leads to embryonic lethality from gastrulation on, similar to BRG1-/- mice. Transcriptome analysis at late blastula, before development becomes arrested, indicates predominantly a role for BRG1 in transcriptional activation of a limited set of genes involved in pattern specification processes and nervous system development. Mosaic analysis by targeted microinjection defines BRG1 as an essential amplifier of gene expression in dorsal (BCNE and Nieuwkoop centers) and ventral (BMP/Vent) signaling centers. By tissue transplantation, we define BRG1-dependent activation of chordin (chrd) transcription in the prospective neural plate (BCNE region) as an essential step in head formation. BRG1-sensitive genes are typically characterized by a robust burst of transcription at MBT. These results define a systemic function for BRG1-containing SWI/SNF chromatin remodelers as a transcriptional amplifier of the gene network that initiates embryonic patterning.
Overall design RNA isolated from X. tropicalis embryos injected radially at the 2-4 cell stage with Brg1 or control morpholinos and cultivated until late blastula stage. 4 replicate experiments with cells from 10 embryos pooled.
Contributor(s) Wagner G, Singhal N, Nicetto D, Straub T, Kremmer E, Rupp RA
Citation(s) 28498870
Submission date May 27, 2016
Last update date Jul 20, 2017
Contact name Tobias Straub
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address GroƟhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
Platforms (1)
GPL10263 [X_tropicalis] Affymetrix Xenopus tropicalis Genome Array
Samples (8)
GSM2180062 control_rep1
GSM2180063 brg1_rep1
GSM2180064 control_rep2
This SubSeries is part of SuperSeries:
GSE82019 BRG1 Chromatin Remodeling ATPase Balances Germ Layer Patterning by Amplifying the Transcriptional Burst at Midblastula Transition
BioProject PRJNA323627

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Supplementary file Size Download File type/resource
GSE82017_RAW.tar 35.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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