GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE8324 Query DataSets for GSE8324
Status Public on Jun 27, 2008
Title CSMA mouse transcriptome analysis project
Organism Mus musculus
Experiment type Expression profiling by MPSS
Summary The mouse Reference Transcriptome Database (MRTD) is the result of a multi-institute initiative funded with Federal Funds from the National Cancer Institute (NCI) and National Institutes of Health (NIH).
When completed, this database will contain quantitative and comprehensive gene expression data obtained from more than one hundred different mouse samples. These data was initially acquired using the Massively Parallel Signature Sequencing (MPSS™) technology invented by Sidney Brenner and reduced to practice by Lynx Therapeutics, Inc. With recent advent of Illumina's Genome Analyzer technology, it was decided that Illumina?s current Genetic Analyzer is better suited for the project by its Clonal Single Molecule Array (CSMA™) technology. It is therefore adapted for the first 14 samples as displayed here. All samples are and will be sequenced at the Illumina facility in Hayward, CA. The data will be maintained and hosted by both the National Center for Biotechnology Information (NCBI->GEO).
The high homology between human and mouse, the wealth of biological information available, the clinical relevance of the disease models, the completion of the genome sequencing, and the ease of manipulation of the genome make mouse the prime animal model for biomedical research. All these factors influenced the decision to choose this small mammalian as the first transcriptome to be sampled to this unprecedented depth. Eventually this database will contain a large collection of normal tissues or cell types that will serve as the basis for further disease state studies.
CSMA™ is the ideal technology for establishing such a reference database. It provides superior sensitivity and dynamic range and it is the only technology that can routinely provide the sampling depth (2 to 5 million tags per sample) needed for accurately and quantitatively determining the expression level of every gene in a particular sample. In this database each individual sample has been sequenced to a depth of at least 2 million 20 nucleotide long expressed sequence tags.
Unlike most other gene expression technologies that provide analog expression data, CSMA™ provides digital expression information, which is crucial for data exchange, comparison and expansion.
Our goal is to enable researchers to directly access accurate expression information without conducting additional bench work. The database should also be useful for investigators interested in comparing their own set of CSMA™ data to the healthy tissues made available here. Because of the additive nature of this database, researchers will be able to gain insights on the expression levels of their gene(s) of interest across nearly a hundred samples while they will only need to conduct a few additional experiments targeted to their own area of interest.
Keywords: Illumina Genetic Analyzer Sequencing, Clonal Single Molecule Array
Overall design 14 libraries; 7 tissues from male and female
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 28, 2007
Last update date Mar 17, 2012
Contact name Daixing Zhou
Phone 510-670-9441
Fax 510-670-9302
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
Platforms (1)
GPL5449 CSMA [DpnII: Mus musculus] signature list
Samples (14)
GSM206364 Bone marrow, Female (MoBM.Feml)
GSM206365 Bone marrow, Male (MoBM.Male)
GSM206366 Brain Hippocampus, Female (MoBr.FeHi)
BioProject PRJNA101311

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8324_RAW.tar 48.3 Mb (http)(custom) TAR (of TXT)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap