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Series GSE8334 Query DataSets for GSE8334
Status Public on Dec 31, 2012
Title Vitamin D3 affects TNFa mediated activation of dendritic cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary The ability of dendritic cells (DC) to initiate immunity and induce antigen-specific tolerance makes DC ideal targets for pharmacological intervention into immune responses. NF-kB factors are a family of transcriptional regulators important for DC development and function. However, the identity of NF-kB target genes that are central to DC biology is largely unknown. In the present study inhibition of the NF-kB activation by the IkBa super repressor (IkBa-SR) and DNA microarray analysis were used to determine the repertoire of NF-kB responsive genes in DC.

A number of immunomodulatory compounds have been suggested to target the NF-kB signalling pathway and/or NF-kB-mediated transcription of pro-inflammatory target genes. 1a,25-dihydroxyvitamin D3 (VD3) exerts its effects through the vitamin D3 receptor (VDR), a member of the nuclear hormone receptor superfamily. Microarray analysis was also selected as an approach of choice for the analysis of the effects of VD3 on the activation of DC, and to survey the involvement of VDR in repression of NF-kB regulated genes. These genes can be potentially useful targets for the development of more specific anti-inflammatory agents for clinical applications.
Keywords: drug treatment, TNFa treatment, VD3 treatment
Overall design DC were generated in vitro from bone marrow cells of VDRmut and VDRwt control mice as described previously (Hieronymus, T., T. C. Gust, R. D. Kirsch, T. Jorgas, G. Blendinger, M. Goncharenko, K. Supplitt, S. Rose-John, A. M. Muller, and M. Zenke. 2005. Progressive and controlled development of mouse dendritic cells from Flt3+CD11b+ progenitors in vitro. J Immunol 174:2552-2562). Immature DC were pre-treated with 1a,25-dihydroxyvitamin D3 (VD3), or left untreated. TNFa was added where appropriate, and cells incubated further for 4 h. Total RNA was amplified, labelled and hybridised to Affymetrix MOE430A arrays. TNFa up- and down-regulated genes, as well as genes affected by VD3 were analysed.
Contributor(s) Goncharenko M, Kaergel E, Schroeder V, Gust T, Scheidereit C, Zenke M, Hieronymus T
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Submission date Jun 29, 2007
Last update date Jan 08, 2019
Contact name Mykola Goncharenko
Phone +492418080763
Fax +492418082442
Organization name IBMT - Cell Biology, University Hospital, RWTH Aachen
Department Institute for Biomedical Technology - Cell Biology
Street address Pauwelstrasse 30
City Aachen
ZIP/Postal code 52074
Country Germany
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (8)
GSM206416 VDRwt DC - untreated
GSM206648 VDRwt DC - VD3
GSM206649 VDRwt DC - TNFa
BioProject PRJNA101331

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Supplementary file Size Download File type/resource
GSE8334_RAW.tar 164.0 Mb (http)(custom) TAR (of CEL, CHP, EXP, RPT, TIFF, TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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