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Status |
Public on Nov 20, 2016 |
Title |
Dynamic regulation of chromatin signatures during Drosophila embryonic glial differentiation [ChIP-Seq] |
Organism |
Drosophila melanogaster |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Employ affinity purification approach to capture different stages of glial-specific information on gene expression, nucleosome occupancy and histone modifications (HMs) in Drosophila melanogaster embryo
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Overall design |
Cell type-specific nucleosome and histone modification maps were generated from GBs and glias nuclei, which isolated from 5–7h-old gcm-Gal4,UAS-mCDGFP/cyo;UAS-3xFLAG-blrp-mCherry-RanGap and 12–14h-old repo-Gal4,UAS-3xFLAG-blrp-mCherry-RanGap/Tm3B D. melanogaster embryos by the method INTACT (Steinner et al., 2012). We determinated nucleosome occupancy in these nuclei by micrococcal nuclease sequencing (MNase-Seq). We also performed ChIP-Seq (chromatin immunoprecipitation and sequencing) for five histone modifications (H3K4me3, H3K4me1,H3K9ac, H3K27ac, and H3K27me3). Sequencing was performed on Illumina HiSeq 2000 using signle end protocol.
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Contributor(s) |
Ye Y, Jiang C |
Citation(s) |
27634414, 31195140 |
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Submission date |
Jun 15, 2016 |
Last update date |
Nov 12, 2019 |
Contact name |
Youqiong Ye |
E-mail(s) |
youqye@gmail.com
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Phone |
+8613661565591
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Organization name |
Shanghai Jiao Tong University School of Medicine
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Street address |
280 Chongqing south road
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City |
Shanghai |
ZIP/Postal code |
200025 |
Country |
China |
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Platforms (1) |
GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
GSE83377 |
Dynamic regulation of chromatin signatures during Drosophila embryonic glial differentiation |
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Relations |
BioProject |
PRJNA325733 |
SRA |
SRP076594 |