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Series GSE83426 Query DataSets for GSE83426
Status Public on Aug 10, 2016
Title Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary Eukaryotic gene expression requires that RNA Polymerase II (RNAP II) gain access to DNA in the context of chromatin. The C-terminal domain (CTD) of RNAP II recruits chromatin modifying enzymes to promoters, allowing for transcription initiation or repression. Specific CTD phosphorylation marks facilitate recruitment of chromatin modifiers, transcriptional regulators, and RNA processing factors during the transcription cycle. However, the readable code for recruiting such factors is still not fully defined and how CTD modifications affect related families of genes or regional gene expression is not well understood. Here we examine the effects of manipulating the Y1S2P3T4S5P6S7 heptapeptide repeat of the CTD of RNAP II in Schizosaccharomyces pombe by substituting non-phosphorylatable alanines for Ser2 and/or Ser7 and the phosphomimetic glutamic acid for Ser7. Global gene expression analyses were conducted using splicing-sensitive microarrays and validated via RT-qPCR. The CTD mutations did not affect pre-mRNA splicing or snRNA levels. Rather, the data revealed upregulation of subtelomeric genes and alteration of the repressive histone H3 lysine 9 methylation (HeK9me) landscape. The data further indicate that H3K9me and expression status are not fully correlated, suggestive of CTD-dependent subtelomeric repression mechansims that act independently of H3K9me levels.
 
Overall design Splicing sensitive S. pombe microarrays (Agilent-027365) were used to compare the splicing and expression profile of four mutant strains relative to WT control with 3-5 biological replicates and dye flipped samples
 
Contributor(s) Pleiss JA, Inada M
Citation(s) 27402158
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 GM098634 Mechanisms of environmentally responsive splicing in S.Pombe Cornell University JEFFREY A PLEISS
Submission date Jun 16, 2016
Last update date Aug 10, 2016
Contact name Jeffrey A Pleiss
E-mail(s) jpleiss@cornell.edu
Organization name Cornell University
Department Molecular Biology and Genetics
Street address 451 Biotech
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platforms (1)
GPL21587 Agilent-027365 Schizosaccharomyces pombe V3 [Probe Name version]
Samples (16)
GSM2202914 S7A_rep1
GSM2202915 S7A_rep2
GSM2202916 S7A_rep3
Relations
BioProject PRJNA325877

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Supplementary file Size Download File type/resource
GSE83426_RAW.tar 23.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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