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| Status |
Public on Feb 08, 2017 |
| Title |
Renal fibrosis mRNA classifier: validation in experimental lithium-induced interstitial fibrosis in the rat kidney |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Metzincins and related genes (MARGS) play important roles in ECM remodeling in fibrotic conditions.
In this investigation, gene expression was examined in a rat model to investigate whether the previously described MARGS based fibrosis classifier had diagnostic value in an experimental rat model of lithium-induced renal fibrosis
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| Overall design |
Male Wistar rats (n=12) were divided into 2 groups (n=6) One group was given a diet containing lithium (40 mmol/kg food for 7 days, followed by 60mmol/kg food for the rest of the experimental period, while a control group (n=6) was fed a normal diet. After six months, animals were sacrificed and the renal cortex and medulla of both kidneys removed for analysis. For microarray investigation, 24 GeneChip® Affymetrix Rat Exon 1.0 ST arrays were used. Statistically relevant genes (p-value<0.05, fold change>1.5, t-test) were further examined.
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| Contributor(s) |
Jeffs A, Scherer A, Leader J, Leader C, Bedford J, Marti H, Walker R |
| Citation(s) |
28002484 |
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| Submission date |
Jun 22, 2016 |
| Last update date |
Feb 08, 2017 |
| Contact name |
Philipp Strauss |
| E-mail(s) |
Philipp.Strauss@uib.no
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| Organization name |
University of Bergen
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| Street address |
Haukeland Universitetssykehus Laboratoriebygget, 7. etg. Heis øst
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| City |
Bergen |
| ZIP/Postal code |
5020 |
| Country |
Norway |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA326499 |
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