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Series GSE84081 Query DataSets for GSE84081
Status Public on Feb 04, 2022
Title Contribution of host an viral small non-coding RNAs to SARS-CoV lung pathology
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Severe acute respiratory syndrome coronavirus (SARS-CoV) causes lethal disease in humans, with viral E protein promoting the exacerbated inflammatory response. By deep sequencing RNAs from the lungs of infected mice, we have addressed the relevance of small, non-coding RNAs in SARS-CoV pathology. Host microRNAs (miRNAs) expressed during infection by a virulent virus encoding the E protein were significantly enriched for cytokine-mediated inflammatory pathways when compared with attenuated SARS-CoV-∆E, suggesting contribution of miRNAs to E protein-induced inflammation. The discovery of three 18-22 nt small viral RNAs (svRNAs) derived from the nsp3 and N genomic regions of SARS-CoV in mouse lung and cell cultures is also described. Depletion of these svRNAs significantly reduced viral titers and genomic RNA levels, indicating their positive contribution to virus growth. Remarkably, svRNA-N antagomirs significantly reduced in vivo lung pathology and pro-inflammatory cytokine expression, indicating that svRNAs contribute to SARS-CoV pathogenesis and highlighting the potential of these antagomirs as antivirals.
Overall design Strand-specific, single-end, reads were generated for detecting smallRNAs (18 nts or more) in lungs (mouse). Three or four replicates were prepared per sample type.
Contributor(s) Morales L, Oliveros JC, Fernández-Delgado R, tenOever BR, Enjuanes L, Sola I
Citation(s) 35229638
Submission date Jul 06, 2016
Last update date Mar 31, 2022
Contact name Juan Carlos Oliveros
Organization name CNB, CSIC
Street address Darwin 3
City Cantoblanco
State/province Madrid
ZIP/Postal code 28049
Country Spain
Platforms (1)
GPL15103 Illumina HiSeq 1000 (Mus musculus)
Samples (16)
GSM2226897 Infected (deltaE) - 2dpi - Rep1
GSM2226898 Infected (deltaE) - 2dpi - Rep2
GSM2226899 Infected (deltaE) - 2dpi - Rep3
BioProject PRJNA328001
SRA SRP077981

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Supplementary file Size Download File type/resource
GSE84081_read_counts.txt.gz 163.6 Kb (ftp)(http) TXT
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