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Series GSE84209 Query DataSets for GSE84209
Status Public on Jun 30, 2017
Title BET inhibitors suppress BZLF1 expression [MutuI RNA-seq]
Organisms Homo sapiens; human gammaherpesvirus 4
Experiment type Expression profiling by high throughput sequencing
Summary Lytic infection by the Epstein-Barr virus (EBV) poses numerous health risks, such as infectious mononucleosis and lymphoproliferative disorder. We demonstrate that JQ1 and other BET inhibitors block two different steps in the sequential cascade of the EBV life cycle: expression of the immediate-early gene BZLF1 and lytic genome replication. This represents a novel mode of action for antiviral drugs that may increase efficacy and decrease emergence of resistance. The RNA-seq data below show that the first block by JQ1 occurs before transcription of any EBV lytic genes (see wigs of EBV transcriptomes). A subset of the same RNA-seq data (three replicates each of Mutu+vehicle was compared to Mutu+JQ1) was examined for changes in the human transcriptome, and those results are presented in the spreadsheet "Differential_Expression_GEO"
 
Overall design RNA-seq from MutuI pretreated with JQ1 or vehicle, treated with antibody or vehicle, experiment performed in triplicate
 
Contributor(s) Keck KM, Miranda JL
Citation(s) 28588024
Submission date Jul 10, 2016
Last update date May 15, 2019
Contact name JJ Miranda
E-mail(s) jmiranda@barnard.edu
Organization name Barnard College, Columbia University
Street address 3009 Broadway
City New York
ZIP/Postal code 10027
Country USA
 
Platforms (1)
GPL22131 Illumina HiSeq 2500 (Homo sapiens; Human herpesvirus 4)
Samples (12)
GSM2229408 RNA-seq_MutuI+vehicle_Replicate1
GSM2229409 RNA-seq_MutuI+vehicle_Replicate2
GSM2229410 RNA-seq_MutuI+vehicle_Replicate3
This SubSeries is part of SuperSeries:
GSE84214 BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps
Relations
BioProject PRJNA328391
SRA SRP078214

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84209_Differential_Expression_GEO.xlsx 17.9 Mb (ftp)(http) XLSX
GSE84209_RAW.tar 11.8 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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