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Series GSE84211 Query DataSets for GSE84211
Status Public on Jun 30, 2017
Title BET inhibitors suppress lytic DNA replication [Akata-Zta RNA-seq]
Organisms Homo sapiens; human gammaherpesvirus 4
Experiment type Expression profiling by high throughput sequencing
Summary Lytic infection by the Epstein-Barr virus (EBV) poses numerous health risks, such as infectious mononucleosis and lymphoproliferative disorder. We demonstrate that JQ1 and other BET inhibitors block two different steps in the sequential cascade of the EBV life cycle: expression of the immediate-early gene BZLF1 and lytic genome replication. This represents a novel mode of action for antiviral drugs that may increase efficacy and decrease emergence of resistance. The RNA-seq data below show that the second block causes a decrease in expression of late but not early lytic genes.
 
Overall design (A) RNA-seq from Akata-Zta pretreated with JQ1 or vehicle, then treated with doxycycline or vehicle, experiment performed in triplicate. (B) RNA-seq from Akata-Zta pretreated with I-BET, then treated with doxycycline or vehicle, experiment performed in triplicate.
 
Contributor(s) Keck KM, Miranda JL
Citation(s) 28588024
Submission date Jul 10, 2016
Last update date May 15, 2019
Contact name JJ Miranda
E-mail(s) jmiranda@barnard.edu
Organization name Barnard College, Columbia University
Street address 3009 Broadway
City New York
ZIP/Postal code 10027
Country USA
 
Platforms (1)
GPL22132 Illumina HiSeq 4000 (Homo sapiens; Human herpesvirus 4)
Samples (18)
GSM2229432 A_RNA-seq_AkataZta+vehicle_Replicate1
GSM2229433 A_RNA-seq_AkataZta+vehicle_Replicate2
GSM2229434 A_RNA-seq_AkataZta+vehicle_Replicate3
This SubSeries is part of SuperSeries:
GSE84214 BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps
Relations
BioProject PRJNA328406
SRA SRP078261

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Supplementary file Size Download File type/resource
GSE84211_RAW.tar 15.7 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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