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| Status |
Public on Aug 15, 2007 |
| Title |
Micro-RNAs in Adult Long-Evans (Turku/AB) Rat Liver are Refractory to 96 Hours Dioxin Treatment |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Dioxin-like chemicals are well-known for their ability to upregulate expression of numerous genes via the AH receptor (AHR). However, recent transcriptomic analyses in several laboratories indicate that dioxin-like chemicals or AHR genotype itself also can downregulate levels of mRNAs encoded by numerous genes. The mechanism responsible for such downregulation is unknown. We hypothesized that microRNAs (miRNAs), which have emerged as powerful negative regulators of mRNA levels in several systems, might be responsible for mRNA downregulation in dioxin/AHR pathways. We used the Exiqon miRNA array platform as well as quantitative RT-PCR to measure miRNA levels in dioxin-sensitive Long-Evans (Turku/AB; L-E) rats. Treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) for 96 hr in vivo caused few changes in miRNA levels in rat livers and those changes that were statistically significant were of modest magnitude.
Feed-restricted-control L-E rats were included to ensure that changes in miRNA levels were due to TCDD-treatment per se and not the result of the decreased feed intake which occurs in dioxin-sensitive strains within 96 h after TCDD exposure.
Manuscript Submitted: Moffat ID, Boutros PC, Celius T, Pohjanvirta R & Okey AB. Micro-RNAs in rodent liver are refractory to dioxin treatment. Toxicological Sciences May, 2007.
Keywords: miRNA expression, response to xenobiotics, feed restriction response
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| Overall design |
A loop design used to profile miRNA levels from dioxin-sensitive L-E AHRWT/WT vehicle-control rats (LC96), those exposed to TCDD for 96 h (LT96), and feed-restricted (LF96) rats.
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| Contributor(s) |
Moffat ID, Boutros PC, Celius T, Lindén J, Pohjanvirta R, Okey AB |
| Citation(s) |
17698510 |
| |
| Submission date |
Jul 13, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Ivy Moffat |
| E-mail(s) |
ivy.moffat@utoronto.ca
|
| Phone |
416-978-2207
|
| Fax |
416-978-6395
|
| Organization name |
University of Toronto
|
| Department |
Pharmacology
|
| Lab |
Dr. Allan B. Okey
|
| Street address |
1 King's College Circle, Medical Science Building Rm.4302
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 1A8 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL5531 |
Exiqon-Rat/Mouse/Human-540-v8.0 |
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| Samples (6)
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| GSM210154 |
L-E rat liver: control vs feed restricted |
| GSM210155 |
L-E rat liver: feed restricted vs TCDD treated |
| GSM210156 |
L-E rat liver: TCDD treated vs contol |
| GSM210157 |
L-E rat liver: control vs TCDD treated |
| GSM210158 |
L-E rat liver: TCDD treated vs feed restricted |
| GSM210159 |
L-E rat liver: feed restricted vs control |
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| This SubSeries is part of SuperSeries: |
| GSE8474 |
MicroRNAs in adult rodent liver and response to dioxin |
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| Relations |
| BioProject |
PRJNA105357 |
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