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Series GSE84763 Query DataSets for GSE84763
Status Public on Aug 25, 2017
Title Differential gene expression in ATZ-globule-free and ATZ-globule-containing hepatocytes in PIZ mouse liver
Organism Mus musculus
Experiment type Expression profiling by array
Summary Alpha-1-antitrypsin deficiency is a disease of the liver and lung where accumulation of misfolded ATZ protein forms large protein aggregates or globules that result in significant cellular stess. From Birth to 2 months of age, virtually every hepatocyte has numerous globules of the mutant ATZ protien. At 2 months of age small colonies of globule free hepatocytes arise and over time the liver becomes largely globule free. this analysis aims to identify differential gene expression profiles in the globule free vs the globule containing cells to idenitfy key factors and pathways regulating clearance of the mutant ATZ protien.
We used Laser Capture Microdisection to isolate RNA from ATZ-globule-free hepatocytes and ATZ-globule-containing hepatocytes and subjected them to microarray analysis to detail the differential global gene expression profiles.
 
Overall design PIZ mice 3 months of age were sacrificed and their livers were mounted in OCT compound and fresh frozen in methylbutane. Liver sections were placed on slides and counterstained with Cresyl Violet to visualize globules. The slides were then subjected to laser capture micro-disection of globule free and globule containing areas.
 
Contributor(s) Bell AW, Oertel M, Luo J, Michalopoulos GK
Citation(s) 27938510
Submission date Jul 25, 2016
Last update date Aug 25, 2017
Contact name George K Michalopoulos
E-mail(s) michalopoulosgk@upmc.edu
Organization name University of Pittsburgh
Street address 411 SBST, 200 Lothrop Street
City Pittsburgh
State/province Pennsylvania
ZIP/Postal code 15261
Country USA
 
Platforms (1)
GPL20775 [MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version]
Samples (2)
GSM2249850 Globule-containing hepatocyte areas from PIZ mouse liver (pooled 2 mice)
GSM2249851 Globule-free hepatocyte areas from PIZ mouse liver (pooled 2 mice)
Relations
BioProject PRJNA331089

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84763_RAW.tar 48.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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