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| Status |
Public on Aug 31, 2017 |
| Title |
Exon level expression profile of MCF7 cells with p53β splice variant knocked out 4hr post 20gy irradiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to explore the funciton of p53β splice variant in DNA damage response, we utilized CRISPR-cas9 genome editing technique to specifically knock out this variant in MCF7 cells.
Microarray analysis is then performed to compare gene expression before and after 20gy irradiation in these cells.
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| Overall design |
control and p53βCRISPR cells in triplicate are subjected to 20gy ionizing irradiation (IR) and 4 hrs post IR, cells are collected for RNA extraction and cDNA systhesis and microarray hybridization.
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| |
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| Contributor(s) |
Chen J, Zhang D, Owzar K, Kastan MB |
| Citation(s) |
28288992 |
| |
| Submission date |
Jul 25, 2016 |
| Last update date |
Aug 31, 2017 |
| Contact name |
Michael Barry Kastan |
| E-mail(s) |
michael.kastan@duke.edu
|
| Organization name |
Duke University
|
| Department |
Duke Cancer Institute
|
| Street address |
2424 Erwin Road
|
| City |
Durham |
| State/province |
NORTH CAROLINA |
| ZIP/Postal code |
27705 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17585 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA331177 |
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