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Series GSE8520 Query DataSets for GSE8520
Status Public on Jul 19, 2007
Title LIN-14 mutants
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary A temporal gradient of the novel nuclear protein LIN-14 specifies the timing and sequence of stage-specific developmental events in Caenorhabditis elegans. The profound effects of lin-14 mutations on worm development suggest that LIN-14 directly or indirectly regulates stage-specific gene expression. We show that LIN-14 can associate with chromatin in vivo and has in vitro DNA binding activity. A bacterially expressed C-terminal domain of LIN-14 was used to select DNA sequences that contain a putative consensus binding site from a pool of randomized double-stranded oligonucleotides. To identify candidates for genes directly regulated by lin-14, we employed DNA microarray hybridization to compare the mRNA abundance of C. elegans genes in wild-type animals to that in mutants with reduced or elevated lin-14 activity. Five of the candidate LIN-14 target genes identified by microarrays, including the insulin/insulin-like growth factor family gene ins-33, contain putative LIN-14 consensus sites in their upstream DNA sequences. Genetic analysis indicates that the developmental regulation of ins-33 mRNA involves the stage-specific repression of ins-33 transcription by LIN-14 via sequence-specific DNA binding. These results reinforce the conclusion that lin-14 encodes a novel class of transcription factor.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Keywords: Logical Set
 
Overall design Computed
 
Contributor(s) Hristova M
Citation(s) 16314527
Submission date Jul 18, 2007
Last update date Mar 17, 2012
Organization Stanford Microarray Database (SMD)
E-mail(s) array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (2)
GPL14 ce_rosetta
GPL2646 CE_printK
Samples (6)
GSM211534 N2b (red, channel 2) vs. lin-4b (green, channel 1)
GSM211535 N2 (red, channel 2) vs. lin-14 (n179ts) (green, channel 1)
GSM211536 N2c (red, channel 2) vs. lin-4c (green, channel 1)
Relations
BioProject PRJNA101641

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
Processed data included within Sample table

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