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Series GSE8520 Query DataSets for GSE8520
Status Public on Jul 19, 2007
Title LIN-14 mutants
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary A temporal gradient of the novel nuclear protein LIN-14 specifies the timing and sequence of stage-specific developmental events in Caenorhabditis elegans. The profound effects of lin-14 mutations on worm development suggest that LIN-14 directly or indirectly regulates stage-specific gene expression. We show that LIN-14 can associate with chromatin in vivo and has in vitro DNA binding activity. A bacterially expressed C-terminal domain of LIN-14 was used to select DNA sequences that contain a putative consensus binding site from a pool of randomized double-stranded oligonucleotides. To identify candidates for genes directly regulated by lin-14, we employed DNA microarray hybridization to compare the mRNA abundance of C. elegans genes in wild-type animals to that in mutants with reduced or elevated lin-14 activity. Five of the candidate LIN-14 target genes identified by microarrays, including the insulin/insulin-like growth factor family gene ins-33, contain putative LIN-14 consensus sites in their upstream DNA sequences. Genetic analysis indicates that the developmental regulation of ins-33 mRNA involves the stage-specific repression of ins-33 transcription by LIN-14 via sequence-specific DNA binding. These results reinforce the conclusion that lin-14 encodes a novel class of transcription factor.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Keywords: Logical Set
Overall design Computed
Contributor(s) Hristova M
Citation(s) 16314527
Submission date Jul 18, 2007
Last update date Mar 17, 2012
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL14 ce_rosetta
GPL2646 CE_printK
Samples (6)
GSM211534 N2b (red, channel 2) vs. lin-4b (green, channel 1)
GSM211535 N2 (red, channel 2) vs. lin-14 (n179ts) (green, channel 1)
GSM211536 N2c (red, channel 2) vs. lin-4c (green, channel 1)
BioProject PRJNA101641

Download family Format
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Supplementary data files not provided
Processed data included within Sample table

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